2. Academic Validation
  3. Discovery of Novel Small-Molecule Inhibitors of LIM Domain Kinase for Inhibiting HIV-1

Discovery of Novel Small-Molecule Inhibitors of LIM Domain Kinase for Inhibiting HIV-1

  • J Virol. 2017 Jun 9;91(13):e02418-16. doi: 10.1128/JVI.02418-16.
Fei Yi 1,  Jia Guo 1,  Deemah Dabbagh 1,  Mark Spear 1,  Sijia He 2,  Kylene Kehn-Hall 1,  Jacque Fontenot 1,  Yan Yin 3,  Mathieu Bibian 3,  Chul Min Park 3,  Ke Zheng 3,  Ha Jeung Park 3,  Veronica Soloveva 4 5,  Dima Gharaibeh 4,  Cary Retterer 4,  Rouzbeh Zamani 4,  Margaret L Pitt 4,  John Naughton 6,  Yongjun Jiang 2,  Hong Shang 2,  Ramin M Hakami 1,  Binhua Ling 7,  John A T Young 6,  Sina Bavari 4,  Xuehua Xu 8,  Yangbo Feng 9,  Yuntao Wu 10
Affiliations

Affiliations

  • 1 National Center for Biodefense and Infectious Diseases, George Mason University, Manassas, Virginia, USA.
  • 2 Department of Laboratory Medicine, China Medical University, Shenyang, China.
  • 3 Medicinal Chemistry, Translational Research Institute, The Scripps Research Institute, Jupiter, Florida, USA.
  • 4 U.S. Army Medical Research Institute of Infectious Diseases, Frederick, Maryland, USA.
  • 5 Henry M. Jackson Foundation, Bethesda, Maryland, USA.
  • 6 Nomis Foundation Laboratories for Immunobiology and Microbial Pathogenesis, Salk Institute of Biological Studies, La Jolla, California, USA.
  • 7 Tulane National Primate Research Center, Covington, Louisiana, USA.
  • 8 Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, Rockville, Maryland, USA.
  • 9 Reaction Biology Corporation, Malvern, Pennsylvania, USA yangbof@gmail.com ywu8@gmu.edu.
  • 10 National Center for Biodefense and Infectious Diseases, George Mason University, Manassas, Virginia, USA yangbof@gmail.com ywu8@gmu.edu.
PMID: 28381571 DOI: 10.1128/JVI.02418-16
Abstract

A dynamic actin Cytoskeleton is necessary for viral entry, intracellular migration, and virion release. For HIV-1 Infection, during entry, the virus triggers early actin activity by hijacking chemokine coreceptor signaling, which activates a host dependency factor, cofilin, and its kinase, the LIM domain kinase (LIMK). Although knockdown of human LIM domain kinase 1 (LIMK1) with short hairpin RNA (shRNA) inhibits HIV Infection, no specific small-molecule inhibitor of LIMK has been available. Here, we describe the design and discovery of novel classes of small-molecule inhibitors of LIMK for inhibiting HIV Infection. We identified R10015 as a lead compound that blocks LIMK activity by binding to the ATP-binding pocket. R10015 specifically blocks viral DNA synthesis, nuclear migration, and virion release. In addition, R10015 inhibits multiple viruses, including Zaire ebolavirus (EBOV), Rift Valley fever virus (RVFV), Venezuelan equine encephalitis virus (VEEV), and herpes simplex virus 1 (HSV-1), suggesting that LIMK inhibitors could be developed as a new class of broad-spectrum Antiviral drugs.IMPORTANCE The actin Cytoskeleton is a structure that gives the cell shape and the ability to migrate. Viruses frequently rely on actin dynamics for entry and intracellular migration. In cells, actin dynamics are regulated by kinases, such as the LIM domain kinase (LIMK), which regulates actin activity through phosphorylation of cofilin, an actin-depolymerizing factor. Recent studies have found that LIMK/cofilin are targeted by viruses such as HIV-1 for propelling viral intracellular migration. Although inhibiting LIMK1 expression blocks HIV-1 Infection, no highly specific LIMK inhibitor is available. This study describes the design, medicinal synthesis, and discovery of small-molecule LIMK inhibitors for blocking HIV-1 and several other viruses and emphasizes the feasibility of developing LIMK inhibitors as broad-spectrum Antiviral drugs.

Keywords

CXCR4; Ebola virus; LIM domain kinase; Rift Valley fever virus; Venezuelan equine encephalitis virus; actin; cofilin; cytoskeleton; herpes simplex virus; human immunodeficiency virus.

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