2. Academic Validation
  3. High-Affinity Functional Fluorescent Ligands for Human β-Adrenoceptors

High-Affinity Functional Fluorescent Ligands for Human β-Adrenoceptors

  • Sci Rep. 2017 Sep 26;7(1):12319. doi: 10.1038/s41598-017-12468-3.
Gyuzel Y Mitronova 1,  Gražvydas Lukinavičius 2,  Alexey N Butkevich 3,  Tobias Kohl 4,  Vladimir N Belov 5,  Stephan E Lehnart 4 6,  Stefan W Hell 3 6
Affiliations

Affiliations

  • 1 Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077, Göttingen, Germany. gmitron@gwdg.de.
  • 2 Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077, Göttingen, Germany. glukina@gwdg.de.
  • 3 Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077, Göttingen, Germany.
  • 4 Heart Research Center Göttingen, Department of Cardiology & Pulmonology, University Medical Center Göttingen, Robert-Koch-Str 40, 37075, Göttingen, Germany.
  • 5 Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077, Göttingen, Germany. vbelov@gwdg.de.
  • 6 German Center for Cardiovascular Research (DZHK), partner site Göttingen, Germany.
PMID: 28951558 DOI: 10.1038/s41598-017-12468-3
Abstract

Visualization of the G-protein coupled receptor (GPCR) is of great importance for studying its function in a native cell. We have synthesized a series of red-emitting fluorescent probes targeting β-adrenergic receptor (βAR) that are compatible with confocal and Stimulated Emission Depletion (STED) microscopy as well as with Time-Resolved Fluorescence Resonance Energy Transfer (TR-FRET) binding assay in living cells. The probe based on the agonist BI-167107 and Fluorescent Dye KK114 demonstrates nanomolar binding affinity and up to nine-fold β2AR selectivity over β1AR. Carazolol-derived probes are fluorogenic and allow no-wash imaging experiments. STED microscopy of β2ARs stained at the native expression level on pancreatic CAPAN cells provides two-fold improvement in lateral optical resolution over confocal mode and reveals the formation of receptor microdomains. These probes retain their functional (agonist or antagonist) properties, allowing simultaneous modulation of cyclic adenosine monophosphate (cAMP) levels and receptor internalization as well as imaging receptor localization.

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