1. Academic Validation
  2. Trans-vaccenic acid inhibits proliferation and induces apoptosis of human nasopharyngeal carcinoma cells via a mitochondrial-mediated apoptosis pathway

Trans-vaccenic acid inhibits proliferation and induces apoptosis of human nasopharyngeal carcinoma cells via a mitochondrial-mediated apoptosis pathway

  • Lipids Health Dis. 2019 Feb 9;18(1):46. doi: 10.1186/s12944-019-0993-8.
Jian Song 1 2 Yujie Wang 1 2 Xiaoqin Fan 1 2 Hanwei Wu 1 2 Jinghong Han 3 Ming Yang 4 Lu Lu 1 Guohui Nie 5 6
Affiliations

Affiliations

  • 1 Department of Otolaryngology, Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, 518035, People's Republic of China.
  • 2 Institute of Translational Medicine, Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, 518035, People's Republic of China.
  • 3 Department of Otolaryngology, Peking University Shenzhen Hospital, Shenzhen, 518036, People's Republic of China.
  • 4 Department of Otolaryngology, Shenzhen People's Hospital, The second Affiliated Hospital of Jinan University, Shenzhen, 518000, People's Republic of China.
  • 5 Department of Otolaryngology, Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, 518035, People's Republic of China. nghui@21cn.com.
  • 6 Institute of Translational Medicine, Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, 518035, People's Republic of China. nghui@21cn.com.
Abstract

Background: Intake of trans fatty acids (TFAs) from partially hydrogenated vegetable oil is associated with a variety of adverse outcomes, but little is known about the health effects of ruminant trans fats. Trans-vaccenic acid (TVA) is a naturally occurring TFA found in the fat of ruminants and in human dairy products. The present study was conducted to investigate the Anticancer activity and underlying mechanisms of TVA on human nasopharyngeal carcinoma (NPC) 5-8F and CNE-2 cells.

Methods: A CCK8 assay was used to determine the effect of TVA and the Mcl-1 Inhibitor S63845 on the proliferation of NPC cells. Apoptosis was measured using flow cytometry. Western blotting was used to detect the protein expression levels of factors associated with Bcl-2-family protein signaling and Akt signaling.

Results: TVA significantly inhibited cell proliferation in a dose-dependent manner. Mechanistic investigation demonstrated that TVA significantly decreased p-Akt levels and Bad phosphorylation on Ser-136 and Ser-112. More importantly, we discovered that the Mcl-1 Inhibitor S63845 synergistically sensitized NPC cells to Apoptosis induction by TVA.

Conclusion: TVA can inhibit NPC cell growth and induced Apoptosis through the inhibition of Bad/Akt phosphorylation. The combined use of TVA and Mcl-1 inhibitors offers a potential advantage for nasopharyngeal Cancer treatment.

Keywords

Akt; Apoptosis; Bad; Mcl-1; Nasopharyngeal carcinoma; Trans-vaccenic acid.

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