2. Academic Validation
  3. 4-HNE Induces Apoptosis of Human Retinal Pigment Epithelial Cells by Modifying HSP70

4-HNE Induces Apoptosis of Human Retinal Pigment Epithelial Cells by Modifying HSP70

  • Curr Med Sci. 2019 Jun;39(3):442-448. doi: 10.1007/s11596-019-2057-8.
Lei-Lei Yang 1,  Hao Chen 2,  Jun Wang 3,  Ting Xia 1,  Hong Sun 3,  Chun-Hui Yuan 3,  Shi-Liang Liu 4,  Jian-Bin Chen 5
Affiliations

Affiliations

  • 1 Department of Ophthalmology, Wuhan Fourth Hospital (Puai Hospital), Huazhong University of Science and Technology, Wuhan, 430030, China.
  • 2 Department of Pathology, Zhongnan Hospital of Wuhan University, Wuhan, 430070, China.
  • 3 Department of Laboratory Medicine, Wuhan Medical and Health Center for Women and Children, Huazhong University of Science and Technology, Wuhan, 430016, China.
  • 4 Department of Ophthalmology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
  • 5 Department of Ophthalmology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China. jbchen10@163.com.
PMID: 31209817 DOI: 10.1007/s11596-019-2057-8
Abstract

The role of heat shock protein 70 (HSP70) in Apoptosis of human retinal pigment epithelial cells (ARPE-19) induced by 4-hydroxy-2-nonenal (4-HNE) was explored. Different concentrations of 4-HNE were used to stimulate ARPE-19 cells, and Apoptosis was measured by flow cytometry. The expression of apoptotic-related proteins, HSP70, X-linked inhibitor-of-apoptosis (XIAP), Bcl-2, and Bax were quantified by Western blotting. HSP70 and XIAP overexpression plasmids, or their corresponding siRNAs were transfected into ARPE-19 cells using Lipofectamine™ 2000. Co-immunoprecipitation and Western blotting were used to detect the effect of 4-HNE on the expression of HSP70 and the binding level between 4-HNE and HSP70. The results showed that 4-HNE induced late Apoptosis in ARPE-19 cells, accompanied by elevated levels of 4-HNE-modified HSP70, but it did not affect HSP70 protein expression. 4-HNE-modified HSP70 down-regulated the expression of the Apoptosis inhibitory protein XIAP. Overexpression of HSP70 or XIAP inhibited 4-HNE-induced Apoptosis of ARPE-19 cells. It was suggested that 4-HNE could promote XIAP degradation by modification of HSP70 to induce late Apoptosis of human retinal pigment epithelial cells.

Keywords

4-hydroxy-2-nonenal; HSP70; XIAP; apoptosis; human retinal pigment epithelial cells.

Figures
Products
嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z