2. Academic Validation
  3. Methyl protodioscin from Polygonatum sibiricum inhibits cervical cancer through cell cycle arrest and apoptosis induction

Methyl protodioscin from Polygonatum sibiricum inhibits cervical cancer through cell cycle arrest and apoptosis induction

  • Food Chem Toxicol. 2019 Oct;132:110655. doi: 10.1016/j.fct.2019.110655.
Yi-Long Ma 1,  Ying-Shuo Zhang 2,  Fan Zhang 3,  Yuan-Yuan Zhang 4,  Kiran Thakur 5,  Jian-Guo Zhang 6,  Zhao-Jun Wei 7
Affiliations

Affiliations

  • 1 School of Food Science and Biological Engineering, Hefei University of Technology, Hefei, 230009, People's Republic of China. Electronic address: yilong.ma@hfut.edu.cn.
  • 2 School of Food Science and Biological Engineering, Hefei University of Technology, Hefei, 230009, People's Republic of China. Electronic address: ying_shuo_zhang@163.com.
  • 3 School of Food Science and Biological Engineering, Hefei University of Technology, Hefei, 230009, People's Republic of China. Electronic address: zffs2012@mail.ahnu.edu.cn.
  • 4 School of Food Science and Biological Engineering, Hefei University of Technology, Hefei, 230009, People's Republic of China. Electronic address: 1217461863@qq.com.
  • 5 School of Food Science and Biological Engineering, Hefei University of Technology, Hefei, 230009, People's Republic of China. Electronic address: kumarikiran@hfut.edu.cn.
  • 6 School of Food Science and Biological Engineering, Hefei University of Technology, Hefei, 230009, People's Republic of China. Electronic address: zhangjianguo@hfut.edu.cn.
  • 7 School of Food Science and Biological Engineering, Hefei University of Technology, Hefei, 230009, People's Republic of China; Anhui Province Key Laboratory of Functional Compound Seasoning, Anhui Qiangwang Seasoning Food Co., Ltd, Jieshou, 236500, People's Republic of China. Electronic address: zjwei@hfut.edu.cn.
PMID: 31271762 DOI: 10.1016/j.fct.2019.110655
Abstract

Methyl protodioscin (MPD) is a steroid saponin which has been well known for its pharmacological properties. Herein, we evaluated the anti-cancer activity of MPD for proliferation inhibition and Apoptosis induction in Hela cells. MPD was purified from the rhizoma of Polygonatum sibiricum primarily and identified by HPLC, UPLC-TOF-MS/MS and NMR analysis, respectively. Results showed that MPD repressed cell proliferation at IC50 of 18.49 μM, altered cell morphology, arrested the cell cycle in G2/M phase, facilitated the generation of intracellular ROS and led to cell Apoptosis in a concentration-dependent manner. Furthermore, MPD treatment promoted death receptor pathway and mitochondrial pathway efficiently. The inhibition of Caspase-8 and Caspase-9 proteins in these pathways abolished the Apoptosis significantly, further demonstrated the mechanism of MPD-induced Apoptosis. These findings offer novel information that MPD may be considered as a possible natural anti-cancerous agent in the form of functional foods or medicinal products.

Keywords

Apoptosis; Cell cycle; Methyl protodioscin; Molecular mechanism; Polygonatum sibiricum.

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