2. Academic Validation
  3. Elamipretide as a potential candidate for relieving cryodamage to human spermatozoa during cryopreservation

Elamipretide as a potential candidate for relieving cryodamage to human spermatozoa during cryopreservation

  • Cryobiology. 2020 Aug;95:138-142. doi: 10.1016/j.cryobiol.2020.03.011.
Hongwei Bai 1,  Yingchun Zhang 1,  Shan Tian 1,  Rui Hu 1,  Yu Liang 2,  Jiangang Gao 2,  Yunshan Wang 3,  Bin Wu 4
Affiliations

Affiliations

  • 1 Reproductive Medicine Department, Jinan Central Hospital, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan, China; Reproductive Medicine Department, Jinan Central Hospital Affiliated Shandong University, Jinan, China.
  • 2 School of Life Science, Shandong University, Jinan, China.
  • 3 Reproductive Medicine Department, Jinan Central Hospital, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan, China; Reproductive Medicine Department, Jinan Central Hospital Affiliated Shandong University, Jinan, China; Cheeloo College of Medicine, Shandong University, Jinan, China.
  • 4 Reproductive Medicine Department, Jinan Central Hospital, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan, China; Reproductive Medicine Department, Jinan Central Hospital Affiliated Shandong University, Jinan, China; Cheeloo College of Medicine, Shandong University, Jinan, China. Electronic address: wb0538@163.com.
PMID: 32240641 DOI: 10.1016/j.cryobiol.2020.03.011
Abstract

As a technique widely used in assisted reproduction, human spermatozoa cryopreservation makes it possible to conserve functional sperm for a long time, but the impact of cryodamage on sperm during the process could not be ignored. The objective of the present study was to investigate the efficacy of Elamipretide, a novel small mitochondrial targeting short cytoprotective peptide, in attenuating cryodamage during spermatozoa cryopreservation. Semen samples were collected and cryopreserved in freeze solution containing different concentrations (0.0, 0.1, 1, and 10 μM) of Elamipretide. Sperm motility, viability, membrane integrity, mitochondrial membrane potential, DNA fragmentation, antioxidant profiles, and acrosome reaction were measured and analyzed. The results showed that supplementation of the freeze media with Elamipretide (1 and 10 μM) significantly improved post-thaw sperm parameters including motility and viability, stability of the plasma membrane, and mitochondria and chromosomes. In addition, by adding Elamipretide, excessive oxidation and acrosome dysfunction in sperm cells undergoing freeze-thaw were also significantly attenuated. Therefore, Elamipretide may be a potential candidate for relieving cryodamage to human spermatozoa during cryopreservation.

Keywords

Cryopreservation; Elamipretide; Mitochondria; Sperm.

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