2. Academic Validation
  3. W2476 represses TXNIP transcription via dephosphorylation of FOXO1 at Ser319

W2476 represses TXNIP transcription via dephosphorylation of FOXO1 at Ser319

  • Chem Biol Drug Des. 2021 May;97(5):1089-1099. doi: 10.1111/cbdd.13828.
Li Zhong 1 2,  Qing Liu 1,  Qiaofeng Liu 3,  Shikai Zhang 4,  Yongbing Cao 4,  Dehua Yang 1,  Ming-Wei Wang 1 2 3
Affiliations

Affiliations

  • 1 The National Center for Drug Screening, CAS Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences (CAS), Shanghai, China.
  • 2 University of Chinese Academy of Sciences, Beijing, China.
  • 3 School of Pharmacy, Fudan University, Shanghai, China.
  • 4 Shanghai TCM-Integrated Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
PMID: 33560565 DOI: 10.1111/cbdd.13828
Abstract

Thioredoxin-interacting protein (TXNIP) overexpression is implicated in the pathogenesis of type 2 diabetes. Previous studies have shown that a small molecule compound (W2476) was able to improve β-cell dysfunction and exert therapeutic effects in diabetic mice via repression of TXNIP signaling pathway. The impact of W2476 on TXNIP transcription was thus investigated using the chromatin immunoprecipitation method. It was found that W2476 promotes competitive binding of forkhead box O1 transcription factor (FOXO1) to the carbohydrate response element (ChoRE) sequence associated with ChoRE-binding protein (ChREBP)/Mlx interacting protein-like(Mlx) complexes. This interaction hinders the attachment of Histone Acetyltransferase p300 and reduces histone H4 acetylation on the TXNIP promoter, leading to decreasing TXNIP transcription.

Keywords

TXNIP; W2476; chromatin immunoprecipitation; diabetes; transcription.

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