2. Academic Validation
  3. Low-cost and scalable projected light-sheet microscopy for the high-resolution imaging of cleared tissue and living samples

Low-cost and scalable projected light-sheet microscopy for the high-resolution imaging of cleared tissue and living samples

  • Nat Biomed Eng. 2024 Sep;8(9):1109-1123. doi: 10.1038/s41551-024-01249-9.
Yannan Chen 1 2,  Shradha Chauhan 1,  Cheng Gong 1 2,  Hannah Dayton 1,  Cong Xu 2,  Estanislao Daniel De La Cruz 1,  Yu-Young Wesley Tsai 1 3,  Malika S Datta 1 3,  Gorazd B Rosoklija 4,  Andrew J Dwork 4 5,  J John Mann 4,  Maura Boldrini 4,  Kam W Leong 2,  Lars E P Dietrich 1,  Raju Tomer 6 7 8
Affiliations

Affiliations

  • 1 Department of Biological Sciences, Columbia University, New York, NY, USA.
  • 2 Department of Biomedical Engineering, Columbia University, New York, NY, USA.
  • 3 Mortimer B. Zuckerman Mind Brain and Behavior Institute, Columbia University, New York, NY, USA.
  • 4 Department of Psychiatry, Columbia University Irving Medical Center, New York State Psychiatric Institute, New York, NY, USA.
  • 5 Department of Pathology and Cell Biology, Columbia University, New York, NY, USA.
  • 6 Department of Biological Sciences, Columbia University, New York, NY, USA. raju.tomer@columbia.edu.
  • 7 Department of Biomedical Engineering, Columbia University, New York, NY, USA. raju.tomer@columbia.edu.
  • 8 Mortimer B. Zuckerman Mind Brain and Behavior Institute, Columbia University, New York, NY, USA. raju.tomer@columbia.edu.
PMID: 39209948 DOI: 10.1038/s41551-024-01249-9
Abstract

Light-sheet fluorescence microscopy (LSFM) is a widely used technique for imaging cleared tissue and living samples. However, high-performance LSFM systems are typically expensive and not easily scalable. Here we introduce a low-cost, scalable and versatile LSFM framework, which we named 'projected light-sheet microscopy' (pLSM), with high imaging performance and small device and computational footprints. We characterized the capabilities of pLSM, which repurposes readily available consumer-grade components, optimized optics, over-network control architecture and software-driven light-sheet modulation, by performing high-resolution mapping of cleared mouse brains and of post-mortem pathological human brain samples, and via the molecular phenotyping of brain and blood-vessel organoids derived from human induced pluripotent stem cells. We also report a method that leverages pLSM for the live imaging of the dynamics of sparsely labelled multi-layered Bacterial pellicle biofilms at an air-liquid interface. pLSM can make high-resolution LSFM for biomedical applications more accessible, affordable and scalable.

Figures
Products
嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z