1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. ABHD4 蛋白, Human (sf9, His)

ABHD4 蛋白, Human (sf9, His)

目录号: HY-P76133
产品使用指南

ABHD4 是一种溶血磷脂酶,选择性作用于 N-酰基磷脂酰乙醇胺 (NAPE),这对于 N-酰基磷脂酰乙醇胺(例如 anandamide)的生物合成至关重要。它水解NAPE中的sn-1和sn-2酰基链,生成甘油磷酸-N-酰基乙醇胺(GP-NAE),是生产N-酰基乙醇胺的重要中间体。ABHD4 蛋白, Human (sf9, His) 是重组的 ABHD4 蛋白,由 Sf9 insect cells 表达,带有 N-His 标签。ABHD4 蛋白, Human (sf9, His) 全长 342 个氨基酸,分子量约为 ~40 KDa。

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描述

ABHD4 is a lysophospholipase that acts selectively on N-acylphosphatidylethanolamines (NAPE), which is essential for the biosynthesis of N-acylphosphatidylethanolamines such as anandamide. It hydrolyzes the sn-1 and sn-2 acyl chains in NAPE to generate glycerophosphate-N-acylethanolamine (GP-NAE), which is an important intermediate in the production of N-acylethanolamine. ABHD4 Protein, Human (sf9, His) is the recombinant human-derived ABHD4 protein, expressed by Sf9 insect cells , with N-His labeled tag. The total length of ABHD4 Protein, Human (sf9, His) is 342 a.a., with molecular weight of ~40 KDa.

研究背景

ABHD4 是一种溶血磷脂酶,对 N-酰基磷脂酰乙醇胺 (NAPE) 具有选择性,并在 N-酰基乙醇胺(例如内源性大麻素 anandamide)的生物合成中发挥着至关重要的作用。它通过水解 NAPE 的 sn-1 和 sn-2 酰基链,生成甘油磷酸-N-酰基乙醇胺 (GP-NAE) 来实现这一目标,这是 N-酰基乙醇胺生物合成的关键中间体。ABHD4 在一系列具有饱和、单不饱和和多不饱和 N-酰基链的底物中表现出活性,有助于产生 N-酰基乙醇胺的多样性。值得注意的是,它对其他溶血磷脂(包括溶血磷脂酰胆碱、溶血磷脂酰乙醇胺和溶血磷脂酰丝氨酸)没有显着的活性。

种属

Human

表达系统

Sf9 insect cells

标签

N-His

蛋白编号

Q8TB40 (M1-D342)

基因 ID
蛋白结构
N-term
His
ABHD4 (M1-D342)
Accession # Q8TB40
C-term
同用名
(Lyso)-N-acylphosphatidylethanolamine lipase; Alpha/beta-hydrolase 4; ABHD4
分子量

Approximately 40 kDa.

纯度

Greater than 82% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

ABHD4 Protein, Human (sf9, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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ABHD4 Protein, Human (sf9, His)
目录号:
HY-P76133
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