ACVR2A/Activin RIIA 蛋白, Mouse (HEK293, His)

ACVR2A, also known as Activin RIIA, is an activin type II receptor. On ligand binding, ACVR2A can forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. ACVR2A is a receptor for Activin A, Activin B and Inhibin A^[1][2]. ACVR2A/Activin RIIA Protein, Mouse (HEK293, His) is produced in HEK293 cells with a C-Terminal His-tag. It consists of 134 amino acids (M1-P134).

ACVR2A 是受体丝氨酸/苏氨酸激酶 TGF-β 家族的 II 型成员。ACVR2A 是激活素 A、激活素 B 和抑制素 A 的受体^[1][2]。
不同物种的 ACVR2A 蛋白的氨基酸序列为非常稳定，由此得出的结论是，在进化过程中，ACVR2A 仅发生了轻微改变，并且在人类和动物中，其功能是相似的。
激活素和 BMP 的信号传导是高度混杂的，因为除了通过 ALK4/7 发出信号之外，激活素 II 型受体（ACVR2A 和 2B）还可以与多种 I 型 BMP 受体 (ALK1/2/3/6) 相互作用，后者也可以与 II 型 BMP 受体 BMPRII 形成复合物。II 型受体磷酸化并激活 I 型受体，I 型受体自身磷酸化，然后结合并激活 SMAD 转录调节因子。ACVR2A 可以与不同的 I 型受体形成复合物，向 Smad2/3 (ALK4) 或 Smad1/5/8（ALK2、ALK3、ALK6）发出信号。不同的 I 型受体竞争与 ACVR2A 结合，这种竞争提供了一种平衡激活素 A 介导的 ALK4 依赖性 Smad2/3 信号传导和 BMP 介导的 ALK2 或 ALK3 依赖性 Smad1/5/8 信号传导之间信号传导的机制。在骨髓瘤细胞中，BMP-6 和 BMP-9 通过 ACVR2A/ACVR2B/ALK2 诱导的 SMAD1/5/8 激活可被激活素 A 治疗抑制^{[1][3]< /sup>。}

Recombinant human ACVR2A (5 μg/mL) inhibits the effects of BMP-9 on INA-6 and IH-1 cells^[1].
Recombinant human ACVR2A (1 μg/mL; 96 hours) blocks the β-cell-proliferative effect of adenoviruses containing mouse Pdx-1 (AdCMV-mPdx-1) treatment^[4].

Recombinant mouse ACVR2A (2.5 μg/.1 mL/mouse, i.v) significantly reduces the parasitemia levels in malarial mice^[2].

Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation.The ED₅₀ for this effect is typically 0.4-3 μg/mL in the presence of 10 ng/mL Recombinant Human Activin A.

• 
  Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation.The ED₅₀ for this effect is 0.7681 μg/mL in the presence of 10 ng/ml Recombinant Human Activin A, corresponding to a specific activity is 1302 U/mg.

Mouse

HEK293

C-His

P27038 (A20-P134)

11480  [NCBI]

AILGRSETQECLFFNANWERDRTNQTGVEPCYGDKDKRRHCFATWKNISGSIEIVKQGCWLDDINCYDRTDCIEKKDSPEVYFCCCEGNMCNEKFSYFPEMEVTQPTSNPVTPKP

Approximately 35 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Oddrun Elise Olsen, et al. Activin A inhibits BMP-signaling by binding ACVR2A and ACVR2B. Cell Commun Signal. 2015 Jun 6;13:27.  [Content Brief]

  [2]. V K Chin, et al. Inhibition of Activin A suppressed tumor necrosis factor-α secretion and improved histopathological conditions in malarial mice. Trop Biomed. 2021 Mar 1;38(1):187-204.  [Content Brief]

  [3]. Szabina Szófia Szilágyi, et al. Competition between type I activin and BMP receptors for binding to ACVR2A regulates signaling to distinct Smad pathways. BMC Biol. 2022 Feb 18;20(1):50.  [Content Brief]

  [4]. Heather L Hayes, et al. A Pdx-1-Regulated Soluble Factor Activates Rat and Human Islet Cell Proliferation. Mol Cell Biol. 2016 Nov 14;36(23):2918-2930.  [Content Brief]