ADAM12 蛋白, Human (HEK293, His)

ADAM12 Protein significantly contributes to skeletal muscle regeneration, particularly in initiating cell fusion. Its multifaceted involvement extends to forming macrophage-derived giant cells (MGC) and differentiating osteoclasts from mononuclear precursors, showcasing a broader role beyond muscle regeneration. ADAM12 Protein, Human (HEK293, His) is the recombinant human-derived ADAM12 protein, expressed by HEK293 , with C-His labeled tag. The total length of ADAM12 Protein, Human (HEK293, His) is 485 a.a., with molecular weight of 27 & 55 & 72 kDa, respectively.

ADAM12 蛋白在骨骼肌再生中发挥着重要作用，特别是在细胞融合过程的启动过程中。此外，它还参与巨噬细胞源性巨细胞 (MGC) 的形成以及破骨细胞从单核前体细胞的分化，突显其在肌肉再生之外的细胞事件中的多方面参与。

Measured by its binding ability in a functional ELISA. Immobilized ADAM12 Protein, Human (HEK293, His) at 10 μg/mL (100 μl/well) can bind mouse FLRG-His with a linear range of 0.31-1.25 μg/mL.

Human

HEK293

C-His

O43184 (R29-S513)

8038  [NCBI]

MAARPLPVSPARALLLALAGALLAPCEARGVSLWNQGRADEVVSASVGSGDLWIPVKSFDSKNHPEVLNIRLQRESKELIINLERNEGLIASSFTETHYLQDGTDVSLARNYTVILGHCYYHGHVRGYSDSAVSLSTCSGLRGLIVFENESYVLEPMKSATNRYKLFPAKKLKSVRGSCGSHHNTPNLAAKNVFPPPSQTWARRHKRETLKATKYVELVIVADNREFQRQGKDLEKVKQRLIEIANHVDKFYRPLNIRIVLVGVEVWNDMDKCSVSQDPFTSLHEFLDWRKMKLLPRKSHDNAQLVSGVYFQGTTIGMAPIMSMCTADQSGGIVMDHSDNPLGAAVTLAHELGHNFGMNHDTLDRGCSCQMAVEKGGCIMNASTGYPFPMVFSSCSRKDLETSLEKGMGVCLFNLPEVRESFGGQKCGNRFVEEGEECDCGEPEECMNRCCNATTCTLKPDAVCAHGLCCEDCQLKPAGTACRDSSNSCDLPEFCTGASPHCPANVYLHDGHS

27&55&72 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.