Afamin/AFM 蛋白, Human (578a.a, HEK293, C-His)

Afamin/AFM protein serves as a carrier of hydrophobic molecules in body fluids and plays an important role in the solubility and activity of lipidated Wnt family members (including WNT1, WNT2B, WNT3, WNT3A, WNT5A, WNT7A, WNT7B, WNT8, WNT9A, and WNT9B). The role of WNT10A and WNT10B. It also binds to vitamin E and may act as a transporter of this vitamin in body fluids, especially when the lipoprotein system is deficient. Afamin/AFM Protein, Human (578a.a, HEK293, C-His) is the recombinant human-derived Afamin/AFM protein, expressed by HEK293 , with C-10*His labeled tag. The total length of Afamin/AFM Protein, Human (578a.a, HEK293, C-His) is 587 a.a., with molecular weight of 80-130 kDa.

Afamin/AFM 蛋白作为体液中疏水性分子的载体，可能在脂化 Wnt 家族成员（包括 WNT1、WNT2B、WNT3、WNT3A、WNT5A、WNT7A、WNT7B、WNT8、WNT9A、 WNT9B、WNT10A 和 WNT10B。此外，它还与维生素 E 结合，并可作为体液中维生素 E 的转运蛋白，特别是在脂蛋白系统不足的情况下。维生素 E 可能参与跨血脑屏障的运输。Afamin 与 Wnt 家族成员形成 1:1 复合物，与一系列 WNT 蛋白相互作用，包括 WNT1、WNT2B、WNT3、WNT3A、WNT5A、WNT7A、WNT7B、WNT8、WNT9A、WNT9B、WNT10A 和 WNT10B。

Measured by its ability to chemoattract bioassay using MC3T3-E1 cells. The ED₅₀ for this effect is 15.01ng/mL, corresponding to a specific activity is 4.657×10⁴ units/mg.

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  Measured by its ability to chemoattract bioassay using MC3T3-E1 cells.The ED₅₀ for this effect is 15.01 ng/mL， corresponding to a specific activity is 4.657×10⁴ units/mg.

Human

HEK293

C-10*His

P43652 (L13-N599)

173  [NCBI]

LFFLTESLTLPTQPRDIENFNSTQKFIEDNIEYITIIAFAQYVQEATFEEMEKLVKDMVEYKDRCMADKTLPECSKLPNNVLQEKICAMEGLPQKHNFSHCCSKVDAQRRLCFFYNKKSDVGFLPPFPTLDPEEKCQAYESNRESLLNHFLYEVARRNPFVFAPTLLTVAVHFEEVAKSCCEEQNKVNCLQTRAIPVTQYLKAFSSYQKHVCGALLKFGTKVVHFIYIAILSQKFPKIEFKELISLVEDVSSNYDGCCEGDVVQCIRDTSKVMNHICSKQDSISSKIKECCEKKIPERGQCIINSNKDDRPKDLSLREGKFTDSENVCQERDADPDTFFAKFTFEYSRRHPDLSIPELLRIVQIYKDLLRNCCNTENPPGCYRYAEDKFNETTEKSLKMVQQECKHFQNLGKDGLKYHYLIRLTKIAPQLSTEELVSLGEKMVTAFTTCCTLSEEFACVDNLADLVFGELCGVNENRTINPAVDHCCKTNFAFRRPCFESLKADKTYVPPPFSQDLFTFHADMCQSQNEELQRKTDRFLVNLVKLKHELTDEELQSLFTNFANVVDKCCKAESPEVCFNEESPKIGN

80-130 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.