Animal-Free GM-CSF 蛋白, Pig (His)

The GM-CSF protein acts as a potent cytokine that coordinates the growth and differentiation of hematopoietic precursor cells of different lineages, including granulocytes, macrophages, eosinophils, and erythrocytes. Structurally, GM-CSF exists as a monomer and its signaling is mediated through a dodecamer complex. Animal-Free GM-CSF Protein, Pig (His) is the recombinant pig-derived animal-FreeGM-CSF protein, expressed by E. coli , with N-His labeled tag. The total length of Animal-Free GM-CSF Protein, Pig (His) is 127 a.a., with molecular weight of ~15.3 kDa.

GM-CSF 蛋白作为一种有效的细胞因子，协调不同谱系的造血前体细胞的生长和分化，包括粒细胞、巨噬细胞、嗜酸性粒细胞和红细胞。在结构上，GM-CSF 作为单体存在，其信号传导通过十二聚体复合物介导。该复合物包含两个头对头的六聚体，每个六聚体具有两个 α、两个 β 和两个配体亚基，强调了受体组装的复杂性以及 GM-CSF 在调节细胞反应中的特异性。

Measure by its ability to induce proliferationin TF-1 cells. The ED₅₀ for this effect is <3 ng/mL.

Pig

E. coli

N-His

Q29118 (A18-K144)

397208  [NCBI]

APTRPPSPVTRPWQHVDAIKEALSLLNNSNDTAAVMNETVDVVCEMFDPQEPTCVQTRLNLYKQGLRGSLTRLKSPLTLLAKHYEQHCPLTEETSCETQSITFKSFKDSLNKFLFTIPFDCWGPVKK

Approximately 15.3 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a solution containing 1X PBS, pH 7.4, trehalose.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.