2. 重组蛋白
  3. Immune Checkpoint Proteins CD Antigens
  4. Inhibitory Checkpoint Molecules Stimulatory Immune Checkpoint Molecules T Cell CD Proteins B Cell CD Proteins NK Cell CD Proteins Macrophage CD Proteins Monocyte CD Proteins Dendritic Cell CD Proteins Endothelial cell CD Proteins
  5. B7-2/CD86
  6. B7-2/CD86 蛋白, Human (HEK293, Fc-His)

B7-2/CD86 蛋白, Human (HEK293, Fc-His)

目录号: HY-P7323
产品使用指南

B7-2/CD86 蛋白, Human (HEK293, Fc-His)是在 HEK293 细胞中产生的 C-末端标记 Fc-His 标签的多肽链。B7-2 (CD86) 是由抗原呈递细胞 (APC) 表达的共刺激分子，与 CD28 相互作用来促进 T 细胞活化和存活，并与 CTLA4 相互作用来进行免疫调节。

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规格 是否有货
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100 μg   询价  

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描述

B7-2/CD86 Protein, Human (HEK293, Fc-His) is a polypeptide chain with the C-termimal human Fc-His Tag produced in HEK293 cells. B7-2 (CD86) is a costimulatory molecule expressed by antigen-presenting cells (APCs), which interacts with CD28 for T-cell activation and survival and with CTLA4 for immune regulation.
研究背景

CD86 是一个跨膜区域，并且具有比 CD80 更长的细胞质结构域。CD86 在叉指树突状细胞 (DC)、朗格汉斯细胞、外周血 DC、记忆 B 细胞和生发中心 B 细胞以及巨噬细胞上组成型表达。通过 Ig 受体交联或添加多种细胞因子激活后，B 细胞上的 CD86 迅速上调[1]。
种属

Human
表达系统

HEK293
标签

C-His
蛋白编号

P42081 (L20-P247)
基因 ID

942  [NCBI]
蛋白结构
N-term
B7-2 (L20-P247)
Accession # P42081
His
C-term
同用名
rHuB7-2/CD86, Fc-His; Activation B7-2 antigen; B70; BU63; CD86; CD28LG2
氨基酸序列

LSGAAPLKIQAYFNETADLPCQFANSQNQSLSELVVFWQDQENLVLNEVYLGKEKFDSVHSKYMGRTSFDSDSWTLRLHNLQIKDKGLYQCIIHHKKPTGMIRIHQMNSELSVLANFSQPEIVPISNITENVYINLTCSSIHGYPEPKKMSVLLRTKNSTIEYDGVMQKSQDNVTELYDVSISLSVSFPDVTSNMTIFCILETDKTRLLSSPFSIELEDPQPPPDHIPHHHHHH

分子量

45-55 kDa
纯度

Greater than 95% as determined by reducing SDS-PAGE.
内毒素含量

<0.1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)
参考文献

B7-2/CD86 Protein, Human (HEK293, Fc-His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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