BCAM 蛋白, Mouse (HEK293, His)

BCAM Protein, acting as a laminin alpha-5 receptor, potentially participates in intracellular signaling, indicated by its resemblance to related proteins. BCAM Protein, Mouse (HEK293, His) is the recombinant mouse-derived BCAM protein, expressed by HEK293 , with C-His labeled tag. The total length of BCAM Protein, Mouse (HEK293, His) is 516 a.a., with molecular weight of 70-90 kDa.

BCAM 蛋白作为层粘连蛋白 α-5 受体发挥作用，并且可能在介导细胞内信号传导中发挥作用，正如其与相关蛋白的相似性所表明的那样。

Measured by the ability of the immobilized protein to support the adhesion of HOS human osteogenic sarcoma cells. When 5x10⁴ cells per well are added to Recombinant Mouse BCAM coated plates, cell adhesion is enhanced in a dose dependent manner. The ED₅₀ for this effect is 0.491 μg/mL. Corresponding to a specific activity is 2.036×10³ U/mg

• 
  Measured by the ability of the immobilized protein to support the adhesion of HOS human osteogenic sarcoma cells. When 5 x 10⁴ cells per well are added to Recombinant Mouse BCAM coated plates, cell adhesion is enhanced in a dose dependent manner. The ED₅₀ for this effect is 0.491 μg/mL, corresponding to a specific activity is 2.036 x 10³ U/mg

Mouse

HEK293

C-His

Q9R069 (E26-A541)

57278  [NCBI]

ELHVSVPPRVEVMRGEQVALDCTPREHPEHYVLEWFLVDGTGARHRLASVEPQGSEFLGTVHSLGRVPPYEVDSRGRLVIAKVQVGDGRDYVCVVKAGAAGTSEATSSVRVFATPEDTEVSPNKGTLSVMDQFAQEIATCSSNNGNPVPRITWYRNGQRLEVPMEVNQKGYITIRTVREASGLYSLTSTLYLRLHKDDRDANFHCAAHYDLPSGQHGRLDSHTFRLTLHYPTEHVEFWVGSPSTTEGWVREGDAVQLLCQGDGSPSPEYSFFRQQGTQEEQLNVNLKGNLTLERVHRNQSGIYGCRVEDYDADEEVQLVKKLKLHVAYLDPLELSVPEELFVFLNSSSTVVNCSARGLPTPTVRWTKDSVTLADGPMLSLQSVTFDSAGTYTCEASTPTVPLLSRTQSFQLIVQGAPELKPNEIMPKSGNSWTEGDEVMLTCSARGFPEPKLTWSQRGDTPAEPPFEGRGWKSSSLMVKVTSALSREGVSCEASNIHGKKGHVFHFGSVAPQTAQA

70-90 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.