BID 蛋白, Human

BID Protein, Human expresses in E. coli. The BH3-only protein BID, a pro-apoptotic member of the Bcl-2 family, was initially discovered through binding to both pro-apoptotic Bax and anti-apoptotic Bcl-2. BID is activated in the BCL-2-regulated or mitochondrial apoptosis pathway and acts as a switch between the extrinsic and intrinsic cell death pathways^[1].

在细胞凋亡过程中，Bid 不仅可以在死亡受体凋亡信号传导过程中被 caspase-8 裂解，还可以被其他 caspase、颗粒酶 B、钙蛋白酶和组织蛋白酶裂解。蛋白酶裂解的 Bid 迁移至线粒体，在线粒体中诱导依赖于促凋亡蛋白 Bax 和/或 Bak 的线粒体外膜的透化，因此 Bid 充当蛋白酶介导的死亡信号的哨兵^[1] 。

Human

E. coli

Tag Free

P55957 (M1-D195)

637  [NCBI]

MDCEVNNGSSLRDECITNLLVFGFLQSCSDNSFRRELDALGHELPVLAPQWEGYDELQTDGNRSSHSRLGRIEADSESQEDIIRNIARHLAQVGDSMDRSIPPGLVNGLALQLRNTSRSEEDRNRDLATALEQLLQAYPRDMEKEKTMLVLALLLAKKVASHTPSLLRDVFHTTVNFINQNLRTYVRSLARNGMD

Approximately 20.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filter solution of 20 mM PB, 100 mM KCl, pH 7.4.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.

• [1]. Billen LP, et al. Bid: a Bax-like BH3 protein. Oncogene. 2008;27 Suppl 1:S93-S104.  [Content Brief]