BLK 蛋白, Human (sf9, GST)

The BLK protein is a key non-receptor tyrosine kinase that is essential for B lymphocyte development and signal transduction. It regulates pro-B to pre-B transition, growth arrest, and apoptosis downstream of B cell receptors by binding and phosphorylating CD79A and CD79B. BLK Protein, Human (sf9, GST) is the recombinant human-derived BLK protein, expressed by Sf9 insect cells , with N-GST labeled tag. The total length of BLK Protein, Human (sf9, GST) is 505 a.a., with molecular weight of ~84 kDa.

BLK 蛋白是一种非受体酪氨酸激酶，在 B 淋巴细胞发育、分化和信号传导中发挥着至关重要的作用。BLK 对于 B 细胞受体 (BCR) 信号转导至关重要，可调节 B 细胞受体下游的 pro-B 到 pre-B 的转变、生长停滞和凋亡。它特异性结合 BCR 复合物的重要组成部分 CD79A 和 CD79B 并使其磷酸化。与 FYN 和 LYN 一起，BLK 有助于前 B 细胞受体介导的 NF-kappa-B 激活，并间接刺激 BTK 分子内自磷酸化。此外，在胰岛中，BLK 调节 β 细胞功能，通过上调 PDX1 和 NKX6-1 来促进胰岛素分泌以响应葡萄糖。此外，BLK 磷酸化 CGAS，影响其亚细胞定位和功能。

Human

Sf9 insect cells

N-GST

P51451 (M1-P505)

640  [NCBI]

MGLVSSKKPDKEKPIKEKDKGQWSPLKVSAQDKDAPPLPPLVVFNHLTPPPPDEHLDEDKHFVVALYDYTAMNDRDLQMLKGEKLQVLKGTGDWWLARSLVTGREGYVPSNFVARVESLEMERWFFRSQGRKEAERQLLAPINKAGSFLIRESETNKGAFSLSVKDVTTQGELIKHYKIRCLDEGGYYISPRITFPSLQALVQHYSKKGDGLCQRLTLPCVRPAPQNPWAQDEWEIPRQSLRLVRKLGSGQFGEVWMGYYKNNMKVAIKTLKEGTMSPEAFLGEANVMKALQHERLVRLYAVVTKEPIYIVTEYMARGCLLDFLKTDEGSRLSLPRLIDMSAQIAEGMAYIERMNSIHRDLRAANILVSEALCCKIADFGLARIIDSEYTAQEGAKFPIKWTAPEAIHFGVFTIKADVWSFGVLLMEVVTYGRVPYPGMSNPEVIRNLERGYRMPRPDTCPPELYRGVIAECWRSRPEERPTFEFLQSVLEDFYTATERQYELQP

Approximately 84 kDa

Greater than 88% as determined by reducing SDS-PAGE

<1 EU/μg, determined by LAL method.