2. 重组蛋白
  3. Enzymes & Regulators
  4. Transferases (EC 2)
  5. BRAF 蛋白, Human (sf9, GST, His)

BRAF 蛋白, Human (sf9, GST, His)

目录号: HY-P702921
COA 产品使用指南

BRAF 蛋白, Human (sf9, GST, His) 是重组的 BRAF 蛋白，由 Sf9 insect cells 表达。

MCE 的所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务

SPR 检测服务   重组蛋白定制服务

1.  客户无需承担相应的运输费用。

2.  同一机构（单位）同一产品试用装仅限申领一次，同一机构（单位）一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 价格 是否有货 数量
10 μg ￥10800 In-stock
50 μg   询价  
100 μg   询价  

* Please select Quantity before adding items.

Top Publications Citing Use of Products

  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

BRAF Protein, Human (sf9, GST, His) is the recombinant human-derived BRAF, expressed by Sf9 insect cells .
生物活性

The BRAF activity was detected using Ultra Luminescence technology. The reaction was performed by incubating the BRAF protein, ATP and substrate at 25°C for 60 min, then adding ADP-Glo reagent and reading RLU signal with BMG.
种属

Human
表达系统

Sf9 insect cells
标签

C-6*His;N-GST
蛋白编号

P15056 (E433-R726)
基因 ID

673
蛋白结构
N-term
GST
BRAF (E433-R726)
Accession # P15056
C-term
同用名
BRAF1; RAFB; Proto-oncogene B-Raf; p94; v-Raf murine sarcoma viral oncogene homolog B1
分子量

Approximately 61.3 kDa
纯度

Greater than 80% as determined by reducing SDS-PAGE
性状

溶液
组分

Supplied as a 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH7.5
复溶方法

Please use rapid thawing with running water to thaw the protein.
保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
运输条件

Shipping with dry ice.
文件资料

COA (209 KB)

产品使用指南 (1213 KB)

BRAF Protein, Human (sf9, GST, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称：

  

* 需求量：

* 客户姓名：

 

* Email：

* 电话：

 

* 公司或机构名称：

   留言给我们：

Bulk Inquiry

Inquiry Information

产品名称：
BRAF Protein, Human (sf9, GST, His)
目录号：
HY-P702921
需求量：

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

   产品名称：

  

* Lot #：

* 客户姓名：

 

* Email：

* 电话：

 

* 公司或机构名称：

   留言给我们：

Request for HNMR Report

We have received your request and will respond to you as soon as possible.

嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z