Calreticulin/CALR 蛋白, Mouse (P.pastoris, His)

The calreticulin/CALR protein acts as a calcium-binding partner and promotes endoplasmic reticulum folding, assembly, and quality control. It interacts with monoglucosylated glycoproteins and mediates nuclear export of the NR3C1 DNA-binding domain. Calreticulin/CALR Protein, Mouse (P.pastoris, His) is the recombinant mouse-derived Calreticulin/CALR protein, expressed by P. pastoris , with N-6*His labeled tag. The total length of Calreticulin/CALR Protein, Mouse (P.pastoris, His) is 399 a.a., with molecular weight of ~48.3 kDa.

钙网蛋白/CALR 蛋白是一种钙结合伴侣，通过钙网蛋白/钙连接蛋白循环协调内质网 (ER) 中的关键功能，促进糖蛋白的折叠、寡聚组装和质量控制。这种凝集素与内质网中合成的几乎所有单葡萄糖基化糖蛋白短暂地相互作用，有助于它们的正常成熟和功能。除了在糖蛋白加工中的作用之外，CALR 还参与多种细胞过程。它与 NR3C1 的 DNA 结合域相互作用，介导其核输出并影响基因表达调控。此外，CALR 可能通过调节钙稳态并参与卵母细胞激活过程中的皮质反应，在卵母细胞成熟中发挥作用，从而可能有助于阻止多精受精。CALR 与多种蛋白质形成复合物，包括 GABARAP、PDIA3/ERp57 和 TRIM21，突出了其在细胞途径中的多功能相互作用。它还与 PPIB、SPACA9 和 CLCC1 进行复杂的蛋白质-蛋白质相互作用，强调了它参与多种细胞过程和蛋白质复合物。

Mouse

P. pastoris

N-6*His

P14211 (D18-L416)

12317  [NCBI]

DPAIYFKEQFLDGDAWTNRWVESKHKSDFGKFVLSSGKFYGDLEKDKGLQTSQDARFYALSAKFEPFSNKGQTLVVQFTVKHEQNIDCGGGYVKLFPSGLDQKDMHGDSEYNIMFGPDICGPGTKKVHVIFNYKGKNVLINKDIRCKDDEFTHLYTLIVRPDNTYEVKIDNSQVESGSLEDDWDFLPPKKIKDPDAAKPEDWDERAKIDDPTDSKPEDWDKPEHIPDPDAKKPEDWDEEMDGEWEPPVIQNPEYKGEWKPRQIDNPDYKGTWIHPEIDNPEYSPDANIYAYDSFAVLGLDLWQVKSGTIFDNFLITNDEAYAEEFGNETWGVTKAAEKQMKDKQDEEQRLKEEEEDKKRKEEEEAEDKEDDDDRDEDEDEEDEKEEDEEESPGQAKDEL

Approximately 48.3 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS,6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.