Calumenin 蛋白, Human (HEK293, His)

Calumenin Protein, Human (HEK293, His), a recombinant human Calumenin produced in HEK293 cells, has a His tag at the N-terminus. Calumenin protein (CP) is located within the endoplasmic reticulum Ca²⁺ binding proteins. Calumenin as a suppressor of cell migration and tumor metastasis. Calumenin binds to and stabilizes fibulin-1, leading to inactivation of extracellular signal-regulated kinases 1 and 2 (ERK1/2) signaling^[1][2].

Calumenin属于CREC蛋白家族，是一种多EF手Ca²⁺结合蛋白，并被发现具有独特的C端SR保留信号HDEF。CP可与兰尼碱受体结合，激活肌浆网Ca²⁺ATP酶(SERCA2a)，调节细胞内钙的释放、钙的摄入和储存，维持钙循环的稳态。CP还可以缓解心肌细胞的ERS并抑制ERS介导的细胞凋亡。CP 可缓解病毒性心肌炎中 ERS 引发的细胞凋亡^[1]。

Human

HEK293

C-6*His

O43852 (K20-F315)

813  [NCBI]

KPTEKKDRVHHEPQLSDKVHNDAQSFDYDHDAFLGAEEAKTFDQLTPEESKERLGKIVSKIDGDKDGFVTVDELKDWIKFAQKRWIYEDVERQWKGHDLNEDGLVSWEEYKNATYGYVLDDPDPDDGFNYKQMMVRDERRFKMADKDGDLIATKEEFTAFLHPEEYDYMKDIVVQETMEDIDKNADGFIDLEEYIGDMYSHDGNTDEPEWVKTEREQFVEFRDKNRDGKMDKEETKDWILPSDYDHAEAEARHLVYESDQNKDGKLTKEEIVDKYDLFVGSQATDFGEALVRHDEFHHHHHH

43-55 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB,150 mM NaCl, pH 7.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Yu Wang, et al. Calumenin relieves cardiac injury by inhibiting ERS-initiated apoptosis during viral myocarditis. Int J Clin Exp Pathol. 2017 Jul 1;10(7):7277-7284.  [Content Brief]

  [2]. Pengli Zheng, et al. Calumenin and fibulin-1 on tumor metastasis: Implications for pharmacology. Pharmacol Res. 2015 Sep;99:11-5.  [Content Brief]