1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3) Cathepsin Carboxypeptidase
  4. Cathepsin A
  5. Cathepsin A 蛋白, Mouse (HEK293, His)

Cathepsin A 蛋白, Mouse (HEK293, His)

目录号: HY-P74346
COA 产品使用指南

组织蛋白酶 A 是一种多功能蛋白质,对 β-半乳糖苷酶和神经氨酸酶活性至关重要。它与这些酶形成保护性结合,确保它们的稳定性和整体功能。组织蛋白酶 A 还表现出羧肽酶活性和使速激肽脱酰胺的能力,突出了其多样化的酶功能。Cathepsin A 蛋白, Mouse (HEK293, His) 是重组的 组织蛋白酶 A 蛋白,由 HEK293 表达,带有 C-10*His 标签。

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规格 价格 是否有货 数量
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5 μg ¥450 In-stock
10 μg ¥750 In-stock
50 μg ¥2150 In-stock
100 μg ¥3600 In-stock
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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

Cathepsin A, a multifunctional protein, crucially supports beta-galactosidase and neuraminidase activities.It forms protective associations with these enzymes, ensuring their stability and overall function.Cathepsin A also displays carboxypeptidase activity and the ability to deamidate tachykinins, highlighting its diverse enzymatic functions.Cathepsin A Protein, Mouse (HEK293, His) is the recombinant mouse-derived Cathepsin A protein, expressed by HEK293 , with C-10*His labeled tag.

研究背景

组织蛋白酶 A 是一种多功能蛋白,在支持 β-半乳糖苷酶和神经氨酸酶的活性中发挥着至关重要的作用。它与这些酶形成关联,提供对其稳定性和整体活性至关重要的保护功能。除了其保护作用外,组织蛋白酶 A 还具有羧肽酶活性,并具有使速激肽脱酰胺的能力,展示了其多种酶功能。

生物活性

Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPPGFSAFK(Dnp)-OH. Read at excitation and emission wavelengths of 320 nm and 405 nm (top read). The specific activity is 82.47 pmol/min/µg, as measured under the described conditions.

种属

Mouse

表达系统

HEK293

标签

C-10*His

蛋白编号

P16675 (A24-Y474)

基因 ID
蛋白结构
N-term
Cathepsin A (A24-Y474)
Accession # P16675
10*His
C-term
中文名
重组鼠Cathepsin A蛋白
同用名
Lysosomal protective protein; CTSA; Cathepsin A; PPCA; Ppgb
氨基酸序列

APDQDEIDCLPGLAKQPSFRQYSGYLRASDSKHFHYWFVESQNDPKNSPVVLWLNGGPGCSSLDGLLTEHGPFLIQPDGVTLEYNPYAWNLIANVLYIESPAGVGFSYSDDKMYVTNDTEVAENNYEALKDFFRLFPEYKDNKLFLTGESYAGIYIPTLAVLVMQDPSMNLQGLAVGNGLASYEQNDNSLVYFAYYHGLLGNRLWTSLQTHCCAQNKCNFYDNKDPECVNNLLEVSRIVGKSGLNIYNLYAPCAGGVPGRHRYEDTLVVQDFGNIFTRLPLKRRFPEALMRSGDKVRLDPPCTNTTAPSNYLNNPYVRKALHIPESLPRWDMCNFLVNLQYRRLYQSMNSQYLKLLSSQKYQILLYNGDVDMACNFMGDEWFVDSLNQKMEVQRRPWLVDYGESGEQVAGFVKECSHITFLTIKGAGHMVPTDKPRAAFTMFSRFLNKEPY

分子量

Approximately 52.8 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of 25 mM Tris, 0.3 M NaCl, pH 8.0.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Cathepsin A Protein, Mouse (HEK293, His)
目录号:
HY-P74346
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