CD28 蛋白, Human/Cynomolgus/Rhesus Macaque (HEK293, C-His)

The CD28 protein is critical for T cell activation, enhancing proliferation, cytokine production, and survival. When linked to TCR/CD3 and CD40L, CD28 promotes the production of IL4 and IL10, thereby regulating immune responses. CD28 Protein, Human/Cynomolgus/Rhesus Macaque (HEK293, C-His) is the recombinant Rhesus Macaque, cynomolgus-derived CD28 protein, expressed by HEK293 , with C-His labeled tag.

CD28 蛋白在 T 细胞激活、促进细胞增殖、细胞因子产生和 T 细胞存活中发挥着关键作用。与 TCR/CD3 和 CD40L 共刺激连接后，CD28 增强 T 细胞中 IL4 和 IL10 的产生，有助于免疫反应调节。此外，CD28 的异构体 3 促进 T 细胞中 CD40L 介导的 NF-κ-B 以及激酶 MAPK8 和 PAK2 的激活。该蛋白通过二硫键形成同型二聚体，并与多种分子相互作用，包括 DUSP14、CD80/B7-1、CD86/B7-2/B70 和 GRB2。同工型 3 与 CD40LG 特异性相互作用，突出了其在介导免疫反应和细胞信号传导途径中的多方面作用。

Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus/Rhesus Macaque CD28 is coated at 1 μg/mL (100 μL/well) can bind Recombinant Human CD80. The ED₅₀ for this effect is 0.1938 μg/mL.

Human; Cynomolgus

HEK293

C-6*His

P10747-1/Q0PDN3/Q0PDN4 (N19-P152)

940/705313

NKILVKQSPMLVAYDNAVNLSCKYSYNLFSREFRASLHKGLDSAVEVCVVYGNYSQQLQVYSKTGFNCDGKLGNESVTFYLQNLYVNQTDIYFCKIEVMYPPPYLDNEKSNGTIIHVKGKHLCPSPLFPGPSKP

Approximately 27-45 kDa due to the glycosylation.

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.