CD40 蛋白, Canine (HEK293, His)

CD40 Protein, the receptor for TNFSF5/CD40LG, transduces signals via TRAF6 and MAP3K8, activating ERK in macrophages and B cells, leading to immunoglobulin secretion. Existing as a monomer and homodimer, CD40 interacts with TRAF proteins (TRAF1, TRAF2, TRAF3, TRAF5, and TRAF6), with its interaction with TRAF6 and MAP3K8 crucial for ERK activation. CD40 Protein, Canine (HEK293, His) is the recombinant canine-derived CD40 protein, expressed by HEK293 , with C-His labeled tag.

CD40 蛋白作为 TNFSF5/CD40LG 的受体，转导 TRAF6 和 MAP3K8 介导的信号，激活巨噬细胞和 B 细胞中的 ERK，最终诱导免疫球蛋白分泌。CD40 蛋白以单体和同二聚体形式存在，与各种 TRAF 蛋白相互作用，包括 TRAF1、TRAF2、TRAF3、TRAF5 和 TRAF6。此外，它与 TRAF6 和 MAP3K8 的相互作用对于 ERK 的激活至关重要。

Immobilized Canine CD40, His Tag at 5 μg/mL (100 μl/well) on the plate. Dose response curve for Human CD40 Ligand (Trimer) , hFc Tag with the EC₅₀ of 6.5 ng/mL determined by ELISA.

Canine

HEK293

C-His

Q7YRL5 (E21-A194)

403469  [NCBI]

30-40 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.