CD8 alpha 蛋白, Human (HEK293, His)

CD8 alpha Protein is expressed on the surface of cytotoxic T cells. It plays a crucial role in immune responses by binding to major histocompatibility complex class I molecules on target cells, enhancing T cell activation and cytotoxicity. CD8 alpha Protein is also involved in immune regulation and tolerance. Understanding its functions can aid in developing immunotherapies and vaccines. CD8 alpha Protein, Human (HEK293, His) is the recombinant human-derived CD8 alpha protein, expressed by HEK293 , with N-6*His labeled tag. The total length of CD8 alpha Protein, Human (HEK293, His) is 161 a.a., with molecular weight of ~28 kDa.

CD8 α 是一种完整的膜糖蛋白，在协调针对外部和内部威胁的免疫反应中发挥着关键作用。在 T 细胞中，它作为 MHC I 类分子：肽复合物的辅助受体，促进对源自胞质蛋白的抗原的识别。CD8 α 同时与抗原呈递细胞 (APC) 上的 T 细胞受体 (TCR) 和 MHC I 类蛋白相互作用，将 Src 激酶 LCK 募集至 TCR-CD3 复合物，启动细胞内信号传导途径，最终导致淋巴因子产生、细胞细胞毒性 T 淋巴细胞 (CTL) 的运动、粘附和激活。这种机制使 CTL 能够识别并消除感染细胞或肿瘤细胞。在 NK 细胞中，细胞表面的 CD8 α 同二聚体有助于生存机制，从而实现多个靶细胞的接合和裂解。此外，CD8 α 同二聚体促进活化淋巴细胞的存活和分化为记忆 CD8 T 细胞。该蛋白与细胞表面的 CD8B 形成二硫键连接的异二聚体，并在各种细胞类型（包括 NK 细胞和外周血 T 淋巴细胞）中形成同二聚体。与 MHC I 类 HLA-A/B2M 二聚体和 LCK 的相互作用，以及它与 HLA-G 的直接相互作用，凸显了 CD8 α 在免疫调节中多种功能的复杂分子关联网络。

Human

HEK293

N-6*His

P01732 (S22-D182)

925  [NCBI]

SQFRVSPLDRTWNLGETVELKCQVLLSNPTSGCSWLFQPRGAAASPTFLLYLSQNKPKAAEGLDTQRFSGKRLGDTFVLTLSDFRRENEGYYFCSALSNSIMYFSHFVPVFLPAKPTTTPAPRPPTPAPTIASQPLSLRPEACRPAAGGAVHTRGLDFACD

Approximately 28 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against solution in PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.