CDK2-CCND1 蛋白, Human (sf9, GST, Flag, His)

CDK2 is an important serine/threonine protein kinase that plays a critical regulatory role in the cell cycle, especially during meiosis. It phosphorylates various substrates (CTNNB1, USP37, p53/TP53, NPM1, CDK7, RB1, BRCA2, MYC, NPAT, EZH2) and coordinates centrosome duplication and initiation of DNA synthesis at the G1-S transition. CDK2-CCND1 Protein, Human (sf9, GST, Flag, His) is the recombinant human-derived CDK2-CCND1, expressed by Sf9 insect cells , with N-His, N-GST, N-Flag labeled tag. ,

CDK2 是一种丝氨酸/苏氨酸蛋白激酶，复杂地调节细胞周期，在减数分裂中发挥着不可或缺的作用，但对于有丝分裂来说却是可有可无的。CDK2 在细胞过程的联系中发挥作用，磷酸化多种底物，包括 CTNNB1、USP37、p53/TP53、NPM1、CDK7、RB1、BRCA2、MYC、NPAT 和 EZH2。它协调关键事件，例如 G1-S 转变时的中心体复制和 DNA 合成起始，调节 G2 进展并微调人类胚胎干细胞中细胞增殖、死亡和 DNA 修复之间的平衡。CDK2 依次被细胞周期蛋白 E 和细胞周期蛋白 A2 激活，调节从 S 期到有丝分裂的转变。值得注意的是，EZH2 的磷酸化与维持 H3K27me3 和表观遗传基因沉默有关。CDK2 的参与延伸到 DNA 损伤反应，它调节 G1-S 期检查点、磷酸化 BRCA2 并调节同源重组依赖性修复。此外，CDK2 参与多种信号传导途径，包括维生素 D 介导的生长抑制、NO 介导的信号传导以及 FOXP3 和 ERCC6 等底物的磷酸化，突出了其在细胞生理学中的多方面作用。

The activity was measured by off-chip mobility shift assay(MSA). The enzyme was incubated with fluorecence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by MSA device. The Km of CDK2-CCND1 for its substrate is 280 μM.

Human

Sf9 insect cells

N-His;N-GST;N-Flag

P24941-1 (M1-L298 )&P24385 (M1-I295 )

1017&595

60.5 kDa&36.5 kDa

Greater than 90% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.2 μm filtered solution of 20 mM HEPES (pH 7.5), 200 mM NaCl, 5% glycerol, 1 mM DTT.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.