Complement C8G 蛋白, Human (His)

C8G/C8 γ is the γ subunit of the C8 protein of the complement system and is mainly localized in brain astrocytes. C8G/C8 γ is a neuroinflammation inhibitor that interacts with S1PR2 and jointly antagonizes the pro-inflammatory activity of S1P in microglia. Complement C8G Protein, Human (His) is the recombinant human-derived Complement C8G protein, expressed by E. coli , with N-6*His labeled tag. The total length of Complement C8G Protein, Human (His) is 182 a.a., with molecular weight of ~22.0 kDa.

C8G/C8 γ 是补体系统 C8 蛋白的 γ 亚基，主要定位于脑星形胶质细胞。C8 蛋白是相互作用形成补体细胞溶解膜攻击复合物 (MAC) 的五个成分（C5b、C6、C7、C8、C9）之一。可与 C5B-7 复合物结合，形成 C5B-8 复合物。C5-B8 结合 C9 并在 C9 聚合中充当催化剂。C8 γ 的独特之处在于它属于小型分泌蛋白的脂质运载蛋白家族，具有结合小型疏水性配体的能力。C8γ 的半胱氨酸残基可与 C8α 的二硫键键合，并且在神经炎症中具有抑制作用。在强烈神经炎症的阿尔茨海默病患者中，脑组织、脑脊液和血浆中的 C8G 水平较高。使用重组 C8G 蛋白可抑制急性和慢性阿尔茨海默病动物模型中的神经胶质过度激活、神经炎症和认知能力下降。S1PR2 是 C8G 的一种新型相互作用蛋白，他们共同拮抗小胶质细胞中 S1P 的促炎作用。

Human

E. coli

N-6*His

AAI13627.1 (Q21-R202)

733  [NCBI]

QKPQRPRRPASPISTIQPKANFDAQQFAGTWLLVAVGSACRFLQEQGHRAEATTLHVAPQGTAMAVSTFRKLDGICWQVRQLYGDTGVLGRFLLQARDARGAVHVVVAETDYQSFAVLYLERAGQLSVKLYARSLPVSDSVLSGFEQRVQEAHLTEDQIFYFPKYGFCEAADQFHVLDEVRR

Approximately 22.0-23.0 kDa

Greater than 90% as determined by reducing SDS-PAGE

溶液

Supplied as a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.