1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. ECE-2 蛋白, Human (HEK293, hFc)

ECE-2 蛋白, Human (HEK293, hFc)

目录号: HY-P75267
产品使用指南

ECE-2蛋白通过催化大内皮素-1转化为内皮素-1,在血管调节中发挥着至关重要的作用,有助于调节血压和血管张力。此外,ECE-2 表现出潜在的甲基转移酶活性,并且可能参与淀粉样蛋白-β 加工,这表明该酶对细胞功能的影响比其在内皮素加工中的既定作用更广泛。ECE-2 蛋白, Human (HEK293, hFc) 是重组的 ECE-2 蛋白,由 HEK293 表达,带有 N-hFc 标签。ECE-2 蛋白, Human (HEK293, hFc) 全长 685 个氨基酸,分子量为 120-130 kDa。

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ECE-2 Protein, Human (HEK293, hFc) 相关产品:

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描述

The ECE-2 protein plays a crucial role in vascular regulation by catalyzing the conversion of large endothelin-1 to endothelin-1, helping to regulate blood pressure and vascular tone. Furthermore, ECE-2 exhibits potential methyltransferase activity and may be involved in amyloid-β processing, suggesting that this enzyme has a broader impact on cellular function than its established role in endothelin processing. ECE-2 Protein, Human (HEK293, hFc) is the recombinant human-derived ECE-2 protein, expressed by HEK293 , with N-hFc labeled tag. The total length of ECE-2 Protein, Human (HEK293, hFc) is 685 a.a., with molecular weight of 120-130 kDa.

研究背景

ECE-2蛋白是一种具有双重功能的必需酶。首先,它在将大内皮素-1 转化为内皮素-1 方面发挥着至关重要的作用,内皮素-1 是一种参与血管张力和血压调节的生物活性肽。除了在内皮素系统中发挥作用外,ECE-2 还表现出潜在的甲基转移酶活性,这意味着它参与分子之间的甲基转移。此外,ECE-2 在淀粉样蛋白-β 加工中也可能发挥作用,表明可能与神经退行性疾病相关过程相关。ECE-2 活性的多方面性质强调了其通过内皮素加工在心血管生理学中的重要性,以及对与神经退行性疾病相关的分子事件的潜在贡献(参考:未提供)。

种属

Human

表达系统

HEK293

标签

N-hFc

蛋白编号

P0DPD8-1 (G199-W883)

基因 ID

110599583  [NCBI]

蛋白结构
N-term
hFc
ECE-2 (G199-W883)
Accession # P0DPD8-1
C-term
同用名
EEF1AKMT4-ECE2 readthrough transcript protein; EEF1AKMT4-ECE2; ECE2
分子量

120-130 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

ECE-2 Protein, Human (HEK293, hFc) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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ECE-2 Protein, Human (HEK293, hFc)
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