ECE-2 蛋白, Human (HEK293, His)

The ECE-2 protein plays a crucial role in vascular regulation by catalyzing the conversion of large endothelin-1 to endothelin-1, helping to regulate blood pressure and vascular tone. Furthermore, ECE-2 exhibits potential methyltransferase activity and may be involved in amyloid-β processing, suggesting that this enzyme has a broader impact on cellular function than its established role in endothelin processing. ECE-2 Protein, Human (HEK293, His) is the recombinant human-derived ECE-2 protein, expressed by HEK293 , with N-His labeled tag.

ECE-2蛋白是一种具有双重功能的必需酶。首先，它在将大内皮素-1 转化为内皮素-1 方面发挥着至关重要的作用，内皮素-1 是一种参与血管张力和血压调节的生物活性肽。除了在内皮素系统中发挥作用外，ECE-2 还表现出潜在的甲基转移酶活性，这意味着它参与分子之间的甲基转移。此外，ECE-2 在淀粉样蛋白-β 加工中也可能发挥作用，表明可能与神经退行性疾病相关过程相关。ECE-2 活性的多方面性质强调了其通过内皮素加工在心血管生理学中的重要性，以及对与神经退行性疾病相关的分子事件的潜在贡献（参考：未提供）。

Measured by its ability to cleave a peptide substrate, Mca-RPPGFSAFK(Dnp)-OH. Read at excitation and emission wavelengths of 320 nm and 405 nm (top read). The specific activity is 6005.388 pmol/min/µg, as measured under the described conditions.

Human

HEK293

N-10*His

P0DPD8-1/NP_055508.3 (G199-W883)

110599583  [NCBI]

GVQYHRDPSHSTCLTEACIRVAGKILESLDRGVSPCEDFYQFSCGGWIRRNPLPDGRSRWNTFNSLWDQNQAILKHLLENTTFNSSSEAEQKTQRFYLSCLQVERIEELGAQPLRDLIEKIGGWNITGPWDQDNFMEVLKAVAGTYRATPFFTVYISADSKSSNSNVIQVDQSGLFLPSRDYYLNRTANEKVLTAYLDYMEELGMLLGGRPTSTREQMQQVLELEIQLANITVPQDQRRDEEKIYHKMSISELQALAPSMDWLEFLSFLLSPLELSDSEPVVVYGMDYLQQVSELINRTEPSILNNYLIWNLVQKTTSSLDRRFESAQEKLLETLYGTKKSCVPRWQTCISNTDDALGFALGSLFVKATFDRQSKEIAEGMISEIRTAFEEALGQLVWMDEKTRQAAKEKADAIYDMIGFPDFILEPKELDDVYDGYEISEDSFFQNMLNLYNFSAKVMADQLRKPPSRDQWSMTPQTVNAYYLPTKNEIVFPAGILQAPFYARNHPKALNFGGIGVVMGHELTHAFDDQGREYDKEGNLRPWWQNESLAAFRNHTACMEEQYNQYQVNGERLNGRQTLGENIADNGGLKAAYNAYKAWLRKHGEEQQLPAVGLTNHQLFFVGFAQVWCSVRTPESSHEGLVTDPHSPARFRVLGTLSNSRDFLRHFGCPVGSPMNPGQLCEVW

Approximately 120-125 kDa due to the glycosylation.

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  Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.