ECSIT 蛋白, Human (His)

ECSIT protein is an adapter protein in Toll-like receptors, IL-1, and antiviral signaling that crucially activates NF-kappa-B by complexing with TRAF6 and TAK1/MAP3K7, leading to TAK1/MAP3K7 activation and subsequent IKK activation. Ubiquitinated ECSIT interacts with RELA and NFKB1 and translocates to induce NF-kappa-B-dependent gene expression. ECSIT Protein, Human (His) is the recombinant human-derived ECSIT protein, expressed by E. coli , with N-His labeled tag.

ECSIT 蛋白在多种信号传导途径中充当衔接蛋白，包括 Toll 样受体 (TLR)、白细胞介素 1 (IL-1) 途径和先天抗病毒诱导信号传导。它通过与 TRAF6 和 TAK1/MAP3K7 形成信号复合物，在激活 NF-kappa-B 中发挥关键作用，最终导致 TAK1/MAP3K7 的激活以及随后的 IKK 激活。泛素化后，ECSIT 与解离的 RELA 和 NFKB1 蛋白相互作用，易位至细胞核并诱导 NF-kappa-B 依赖性基因表达。在先天抗病毒免疫反应的背景下，ECSIT 将模式识别受体 RIGI 和 MDA5/IFIT1 桥接到线粒体的 MAVS 复合体上。此外，ECSIT 促进 MAP3K1 的蛋白水解激活，参与 BMP 信号通路，对于正常胚胎发育至关重要。此外，作为 MCIA 复合体的一部分，ECSIT 有助于线粒体复合体 I 的组装。

Human

E. coli

N-His

Q9BQ95-1 (E246-S431)

51295  [NCBI]

QVPLPKDSTGAADPPQPHIVGIQSPDQQAALARHNPARPVFVEGPFSLWLRNKCVYYHILRADLLPPEEREVEETPEEWNLYYPMQLDLEYVRSGWDNYEFDINEVEEGPVFAMCMAGAHDQATMAKWIQGLQETNPTLAQIPVVFRLAGSTRELQTSSAGLEEPPLPEDHQEEDDNLQRQQQGQS

Approximately 25 kDa.

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.