EGFR 蛋白, Cynomolgus (HEK293, His)

EGFR Protein is a receptor tyrosine kinase that binds to ligands of the EGF family. EGFR Protein is involved in the regulation of NF-kappa-B , RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs signaling pathways. EGFR Protein has calmodulin binding activity, epidermal growth factor binding activity and epidermal growth factor activating receptor activity. EGFR Protein, Cynomolgus (HEK293, His) is the recombinant human-derived EGFR protein, expressed by HEK293 , with C-His labeled tag. The total length of EGFR Protein, Cynomolgus (HEK293, His) is 621 a.a., with molecular weight of ~93.8 kDa.

EGFR 蛋白是一种受体酪氨酸激酶，与 EGF 家族的配体结合，包括 EGF、TGFA/ TGF-alpha、AREG、epigen/EPGN、BTC/betacellulin -纤维素蛋白、表调节蛋白/EREG 和 HBEGF/肝素结合 EGF。这种相互作用启动级联反应，将细胞外信号转化为细胞反应，包括受体同源和/或异源二聚化和关键细胞质残基的自磷酸化。磷酸化受体招募适配蛋白如 GRB2，激活下游信号级联，包括 RAS-RAF-MEK-ERK, PI3激酶-ATK, PLCgamma-PKC 和 STATs 模块。此外，EGFR 可能触发 NF-kappa-B 信号级联，并直接磷酸化 RGS16 等蛋白，激活其 GTPase 活性，可能将 EGF 受体信号传导与 G 蛋白偶联受体信号传导联系起来。此外，EGFR 磷酸化 MUC1，增强其与 SRC 和 CTNNB1/ β -catenin的相互作用。它通过与 CCDC88A/GIV 相互作用，正向调节细胞迁移，将 EGFR 保留在细胞膜配体刺激后，从而促进 EGFR 信号传导，触发细胞迁移。除了其典型功能外，EGFR 还有助于增强学习和记忆能力，并在哺乳动物疼痛信号传导中发挥作用，其异构体 2 可能作为 EGF 作用的拮抗剂。

Measured by its binding ability in a functional ELISA.
1.Immobilized EGF Protein, Human, Recombinant (ECD, hFc Tag) at 2 μg/mL (100 μL/well) can bind EGFR Protein, Cynomolgus, Recombinant (ECD, His Tag), the EC₅₀ is 150-500 ng/mL.
2.Immobilized Anti-EGFR(MK)-IgG1 Antibody (Cetuximab) at 2 μg/mL (100 μL/well) can bind EGFR Protein, Cynomolgus, Recombinant (ECD, His Tag),the EC₅₀ is 2-8 ng/mL.

Cynomolgus

HEK293

C-His

XP_005549616 (L25-S645)

102138724  [NCBI]

MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFEDHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENLQIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQWRDIVSSEFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGRCRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNPEGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGIGIGEFKDTLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVKEITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISDGDVIISGNKNLCYANTINWKKLFGTSSQKTKIISNRGENSCKATGQVCHALCSPEGCWGPEPRDCVSCQNVSRGRECVDKCNILEGEPREFVENSECIQCHPECLPQVMNITCTGRGPDNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEGCARNGPKIPS

Approximately 93.8 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.