EphA7 蛋白, Mouse (HEK293, His)

The EphA7 protein is a receptor tyrosine kinase that participates in bidirectional signaling with GPI-anchored ephrin-A ligands (especially EFNA5). It affects brain development by regulating intercellular adhesion and repulsion, contributing to axonal guidance of corticothalamic and retinal axons. EphA7 Protein, Mouse (HEK293, His) is the recombinant mouse-derived EphA7 protein, expressed by HEK293 , with C-His labeled tag. The total length of EphA7 Protein, Mouse (HEK293, His) is 529 a.a., with molecular weight of 68-77 kDa.

EphA7 蛋白是一种受体酪氨酸激酶，与邻近细胞上 GPI 锚定的肝配蛋白 A 配体结合，启动接触依赖性双向信号传导。该受体参与正向信号传导，而肝配蛋白配体则触发反向信号传导。在肝配蛋白-A 配体中，EFNA5 与 EphA7 特异性相互作用，通过影响细胞间粘附和排斥来调节大脑发育。EphA7 在轴突引导中也发挥着至关重要的作用，确保皮质丘脑和视网膜轴突的正确映射。此外，EphA7 可能通过其促凋亡活性促进大脑发育，而促凋亡活性依赖于 caspase (CASP3)。EphA7 的激活可导致 ERK 信号通路组件的磷酸化，包括 MAP2K1、MAP2K2、MAPK1 和 MAPK3。缺乏激酶结构域的异构体 4 也可能调节异构体 1 的粘附特性。

1.Measured by its binding ability in a functional ELISA. Immobilized Mouse EphA7 at 2 μg/mL (100 μL/well) can bind biotinylated Mouse Ephrin-A4. The ED₅₀ for this effect is ≤45.87 ng/mL.
2.Measured by its binding ability in a functional ELISA. Immobilized Mouse EphA7 at 10 μg/mL (100 μL/well) can bind biotinylated Mouse Ephrin-A4. The ED₅₀ for this effect is ≤21 ng/mL.

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  Measured by its binding ability in a functional ELISA. Immobilized Mouse EphA7 at 10 μg/mL (100 μL/well) can bind biotinylated Mouse Ephrin-A4. The ED₅₀ for this effect is 20.7 ng/mL.

Mouse

HEK293

C-10*His

Q61772 (Q28-I556)

13841  [NCBI]

QAAKEVLLLDSKAQQTELEWISSPPSGWEEISGLDENYTPIRTYQVCQVMEPNQNNWLRTNWISKGNAQRIFVELKFTLRDCNSLPGVLGTCKETFNLYYYETDYDTGRNIRENLYVKIDTIAADESFTQGDLGERKMKLNTEVREIGPLSKKGFYLAFQDVGACIALVSVKVYYKKCWSIVENLAVFPDTVTGSEFSSLVEVRGTCVSSAEEEAENSPRMHCSAEGEWLVPIGKCICKAGYQQKGDTCEPCGRRFYKSSSQDLQCSRCPTHSFSDREGSSRCECEDGYYRAPSDPPYVACTRPPSAPQNLIFNINQTTVSLEWSPPADNGGRNDVTYRILCKRCSWEQGECVPCGSNIGYMPQQTGLEDNYVTVMDLLAHANYTFEVEAVNGVSDLSRSQRLFAAVSITTGQAAPSQVSGVMKERVLQRSVQLSWQEPEHPNGVITEYEIKYYEKDQRERTYSTLKTKSTSASINNLKPGTVYVFQIRAVTAAGYGNYSPRLDVATLEEASGKMFEATAVSSEQNPVI

Approximately 68-77 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.