EphB1 蛋白, Mouse (HEK293, His)

EphB1 protein is a receptor tyrosine kinase that participates in bidirectional signaling with ephrin B ligands (including EFNB1, EFNB2, and EFNB3). It plays a crucial role in retinal axon guidance, neural progenitor cell regulation, dendritic spine maturation, synapse formation, angiogenesis, targeted cell migration, and muscle stem cell maintenance. EphB1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived EphB1 protein, expressed by HEK293 , with C-His labeled tag. The total length of EphB1 Protein, Mouse (HEK293, His) is 522 a.a., with molecular weight of ~65-75 kDa.

EphB1 蛋白是一种受体酪氨酸激酶，可与邻近细胞上的肝配蛋白 B 配体结合，产生接触依赖性双向信号传导。该受体介导正向信号传导和反向信号传导途径。其配体包括 EFNB1、EFNB2 和 EFNB3，在各种细胞过程中发挥重要作用。在神经系统发育过程中，EphB1 调节视网膜轴突引导并与 EFNB2 相互作用。在成人神经系统中，它与 EFNB3 一起调节神经祖细胞的趋化性、增殖和极性。EphB1 还参与树突棘成熟、突触形成、血管生成、靶向细胞迁移和粘附。EFNB1 和其他肝配蛋白 B 配体的激活会激活 MAPK/ERK 和 JNK 信号通路，从而调节细胞迁移和粘附。此外，EphB1 通过促进自我更新和减少激活和分化，对于维持肌肉干细胞（卫星细胞）库非常重要。

Immobilized mouse EPHB1 10 μg/mL (100 μL/well) can bind mouse EFNB1, The ED₅₀ for this effect is 128.3 ng/mL.

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  Immobilized mouse EPHB1 10 μg/mL (100 μL/well) can bind mouse EFNB1, The ED₅₀ for this effect is 128.3 ng/mL.

Mouse

HEK293

C-His

Q8CBF3-1 (M18-L539)

270190  [NCBI]

MEETLMDTRTATAELGWTANPASGWEEVSGYDENLNTIRTYQVCNVFEPNQNNWLLTTFINRRGAHRIYTEMRFTVRDCSSLPNVPGSCKETFNLYYYETDSVIATKKSAFWSEAPYLKVDTIAADESFSQVDFGGRLMKVNTEVRSFGPLTRNGFYLAFQDYGACMSLLSVRVFFKKCPSIVQNFAVFPETMTGAESTSLVIARGTCIPNAEEVDVPIKLYCNGDGEWMVPIGRCTCKPGYEPENSVACKACPAGTFKASQEAEGCSHCPSNSRSPSEASPICTCRTGYYRADFDPPEVACTSVPSGPRNVISIVNETSIILEWHPPRETGGRDDVTYNIICKKCRADRRSCSRCDDNVEFVPRQLGLTECRVSISSLWAHTPYTFDIQAINGVSSKSPFPPQHVSVNITTNQAAPSTVPIMHQVSATMRSITLSWPQPEQPNGIILDYEIRYYEKEHNEFNSSMARSQTNTARIDGLRPGMVYVVQVRARTVAGYGKFSGKMCFQTLTDDDYKSELREQL

Approximately 65-75 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.