Ephrin-B1/EFNB1 蛋白, Mouse (HEK293, Fc-His)

Ephrin-B1/EFNB1 proteins are cell surface ligands of Eph receptors that critically regulate migration, repulsion, and adhesion during neuronal, vascular, and epithelial development. Ephrin-B1/EFNB1 Protein, Mouse (HEK293, Fc-His) is the recombinant mouse-derived Ephrin-B1/EFNB1 protein, expressed by HEK293 , with C-hFc, C-6*His labeled tag. The total length of Ephrin-B1/EFNB1 Protein, Mouse (HEK293, Fc-His) is 200 a.a., with molecular weight of 58-80 kDa.

Ephrin-B1/EFNB1 是 Eph 受体的细胞表面跨膜配体，在神经元、血管和上皮发育过程中介导接触依赖性双向信号传导中发挥着关键作用。它与受体酪氨酸激酶 EPHB1/ELK 具有高亲和力，还与 EPHB2 和 EPHB3 结合。与邻近细胞上的 Eph 受体结合启动对迁移、排斥和粘附至关重要的双向信号传导。此外，EFNB1 参与体外诱导连合轴突/生长锥的塌陷，并可能有助于限制纵向突出轴突的方向。该蛋白通过其 PDZ 结合基序与 GRIP1 和 GRIP2 相互作用，并与 TLE1 结合。此外，EFNB1的胞内结构域肽与ZHX2相互作用，增强ZHX2的转录抑制活性。

Measured in a cell proliferation assay using HUVEC cells. The ED₅₀ for this effect is 27.43 ng/mL, corresponding to a specific activity is 3.65×10⁴ U/mg.

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  Measured in a cell proliferation assay using HUVEC cells. The ED₅₀ for this effect is 27.43 ng/mL, corresponding to a specific activity is 3.65×10⁴ U/mg.

Mouse

HEK293

C-hFc;C-6*His

P52795 (K30-S229)

13641  [NCBI]

KNLEPVSWSSLNPKFLSGKGLVIYPKIGDKLDIICPRAEAGRPYEYYKLYLVRPEQAAACSTVLDPNVLVTCNKPHQEIRFTIKFQEFSPNYMGLEFKKYHDYYITSTSNGSLEGLENREGGVCRTRTMKIVMKVGQDPNAVTPEQLTTSRPSKESDNTVKTATQAPGRGSQGDSDGKHETVNQEEKSGPGAGGGGSGDS

58-80 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.