Erythropoietin receptor/EpoR 蛋白, Human (HEK293, His)

The EPOR Protein functions as a receptor for erythropoietin, mediating erythroblast proliferation and differentiation upon EPO stimulation. The heterodimer triggers the JAK2/STAT5 signaling cascade and, in certain cell types, activates STAT1, STAT3, and the LYN tyrosine kinase. Additionally, isoform EPOR-T acts as a dominant-negative receptor, modulating EPOR-mediated signaling pathways. Erythropoietin receptor/EpoR Protein, Human (HEK293, His) is the recombinant human-derived Erythropoietin receptor/EpoR protein, expressed by HEK293 , with C-6*His labeled tag.

EPOR 作为促红细胞生成素 (EPO) 的受体，在介导 EPO 诱导的成红细胞增殖和分化中发挥着至关重要的作用。受到 EPO 刺激后，异源二聚体的 EPOR 成分发生二聚化，启动 JAK2/STAT5 信号级联反应。在某些细胞类型中，这种异二聚体受体复合物还可以激活 STAT1 和 STAT3，并可能参与 LYN 酪氨酸激酶的激活。值得注意的是，同种型 EPOR-T 作为显性失活受体，调节和减弱 EPOR 介导的信号通路。EPORic 复合物内错综复杂的相互作用强调了其在调节细胞对 EPO 刺激反应中的重要性。

Measured by its ability to inhibit Epo-dependent proliferation of TF-1 human erythroleukemic cells. The ED₅₀ for this effect is 79.98 ng/mL, corresponding to a specific activity is 1.2053×10⁴ units/mg.

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  Measured by its ability to inhibit Epo-dependent proliferation of TF‑1 human erythroleukemic cells. The ED₅₀ for this effect is 79.98 ng/mL, corresponding to a specific activity is 1.2053×10⁴ units/mg.

Human

HEK293

C-6*His

P19235-1 (A25-P250)

2057  [NCBI]

APPPNLPDPKFESKAALLAARGPEELLCFTERLEDLVCFWEEAASAGVGPGNYSFSYQLEDEPWKLCRLHQAPTARGAVRFWCSLPTADTSSFVPLELRVTAASGAPRYHRVIHINEVVLLDAPVGLVARLADESGHVVLRWLPPPETPMTSHIRYEVDVSAGNGAGSVQRVEILEGRTECVLSNLRGRTRYTFAVRARMAEPSFGGFWSAWSEPVSLLTPSDLDP

approximately 32.54 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.