FAP 蛋白, Mouse (HEK293, His)

FAP protein is a cell surface glycoprotein serine protease that regulates tissue remodeling, fibrosis, inflammation, and tumor growth. It cleaves post-proline residues, degrades SERPINF2 and SPRY2, and exhibits dipeptidyl peptidase activity. FAP Protein, Mouse (HEK293, His) is the recombinant mouse-derived FAP protein, expressed by HEK293 , with N-His labeled tag. The total length of FAP Protein, Mouse (HEK293, His) is 736 a.a., with molecular weight of 90-110 kDa.

FAP 蛋白是一种细胞表面糖蛋白丝氨酸蛋白酶，参与多种细胞过程，如组织重塑、纤维化、伤口愈合、炎症和肿瘤生长。它具有肽链内切酶活性，特别是切割脯氨酸后残基，并且可以降解 α-2-抗纤溶酶 SERPINF2 和 SPRY2 等物质。FAP 蛋白还具有二肽基肽酶活性，并且优先选择特定的二肽底物。它与 DPP4、PLAUR 和整合素等其他蛋白质相互作用，促进细胞外基质的细胞周蛋白水解，导致细胞粘附、迁移和侵袭。此外，FAP 蛋白在恶性黑色素瘤的组织重塑、伤口愈合和细胞侵袭中发挥作用。它还通过促进血管生成、胶原纤维降解和细胞凋亡来增强肿瘤生长，同时降低免疫系统的抗肿瘤反应。在黑素细胞中，FAP 蛋白通过独立于其丝氨酸蛋白酶活性来调节细胞增殖和存活，从而充当肿瘤抑制因子。

1.Measured by its ability to convert the substrate benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin (Z-GP-AMC) to Z-Gly-Pro and 7-amino-4-methylcoumarin (AMC). The specific activity is ≥1871.29 pmol/min/µg, as measured under the described conditions.
2.Immobilized Mouse FAP, His Tag at 1 μg/mL (100 μl/well) on the plate. Dose response curve for Anti-FAP Antibody, hFc Tag with the EC₅₀ of 4.6 ng/mL determined by ELISA.

Mouse

HEK293

N-His

P97321-1 (L26-D761)

14089  [NCBI]

LRPSRVYKPEGNTKRALTLKDILNGTFSYKTYFPNWISEQEYLHQSEDDNIVFYNIETRESYIILSNSTMKSVNATDYGLSPDRQFVYLESDYSKLWRYSYTATYYIYDLQNGEFVRGYELPRPIQYLCWSPVGSKLAYVYQNNIYLKQRPGDPPFQITYTGRENRIFNGIPDWVYEEEMLATKYALWWSPDGKFLAYVEFNDSDIPIIAYSYYGDGQYPRTINIPYPKAGAKNPVVRVFIVDTTYPHHVGPMEVPVPEMIASSDYYFSWLTWVSSERVCLQWLKRVQNVSVLSICDFREDWHAWECPKNQEHVEESRTGWAGGFFVSTPAFSQDATSYYKIFSDKDGYKHIHYIKDTVENAIQITSGKWEAIYIFRVTQDSLFYSSNEFEGYPGRRNIYRISIGNSPPSKKCVTCHLRKERCQYYTASFSYKAKYYALVCYGPGLPISTLHDGRTDQEIQVLEENKELENSLRNIQLPKVEIKKLKDGGLTFWYKMILPPQFDRSKKYPLLIQVYGGPCSQSVKSVFAVNWITYLASKEGIVIALVDGRGTAFQGDKFLHAVYRKLGVYEVEDQLTAVRKFIEMGFIDEERIAIWGWSYGGYVSSLALASGTGLFKCGIAVAPVSSWEYYASIYSERFMGLPTKDDNLEHYKNSTVMARAEYFRNVDYLLIHGTADDNVHFQNSAQIAKALVNAQVDFQAMWYSDQNHGISSGRSQNHLYTHMTHFLKQCFSLSD

Approximately 90-110 kDa due to the glycosylation

• 
  Greater than 95% as determined by Tris-Bis PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4 or 50 mM Tris-HCL, 300 mM NaCl, pH 8.0

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.