FOXO3 蛋白, Human (HEK293, His)

The FOXO3 protein is a multifaceted transcriptional activator that regulates apoptosis, autophagy, and post-transcriptional MYC control. It actively regulates autophagy in skeletal muscle and induces autophagy gene expression under starvation conditions. FOXO3 Protein, Human (HEK293, His) is the recombinant human-derived FOXO3 protein, expressed by HEK293 , with C-10*His labeled tag.

FOXO3 是一种转录激活剂，在细胞过程中发挥多方面的作用，包括细胞凋亡和自噬。它作为骨骼肌自噬的正调节因子，在饥饿条件下激活自噬基因的表达。FOXO3 在缺乏生存因子的情况下触发细胞凋亡，尤其是在氧化应激期间。此外，它通过诱导 miR-34b 和 miR-34c 的表达参与 MYC 的转录后调控，从而阻止 MYC 翻译。为了应对代谢应激，FOXO3 易位到线粒体中，促进 mtDNA 转录。此外，当脂质稀缺时，它通过促进 SOX9 表达来调节骨骼祖细胞的软骨形成。FOXO3 也是调节性 T 细胞 (Treg) 分化的关键调节因子，可激活 FOXP3 的表达。在各种情况下，FOXO3 与 SIRT3 和线粒体 RNA 聚合酶 POLRMT 形成复合物，从而发挥其多种功能。与 YWHAB/14-3-3-beta、STK4/MST1 和 PIM1 等蛋白质的相互作用进一步调节 FOXO3 活性，凸显其复杂的调控网络。

Human

HEK293

C-10*His

O43524-1 (M1-G673)

2309

MAEAPASPAPLSPLEVELDPEFEPQSRPRSCTWPLQRPELQASPAKPSGETAADSMIPEEEDDEDDEDGGGRAGSAMAIGGGGGSGTLGSGLLLEDSARVLAPGGQDPGSGPATAAGGLSGGTQALLQPQQPLPPPQPGAAGGSGQPRKCSSRRNAWGNLSYADLITRAIESSPDKRLTLSQIYEWMVRCVPYFKDKGDSNSSAGWKNSIRHNLSLHSRFMRVQNEGTGKSSWWIINPDGGKSGKAPRRRAVSMDNSNKYTKSRGRAAKKKAALQTAPESADDSPSQLSKWPGSPTSRSSDELDAWTDFRSRTNSNASTVSGRLSPIMASTELDEVQDDDAPLSPMLYSSSASLSPSVSKPCTVELPRLTDMAGTMNLNDGLTENLMDDLLDNITLPPSQPSPTGGLMQRSSSFPYTTKGSGLGSPTSSFNSTVFGPSSLNSLRQSPMQTIQENKPATFSSMSHYGNQTLQDLLTSDSLSHSDVMMTQSDPLMSQASTAVSAQNSRRNVMLRNDPMMSFAAQPNQGSLVNQNLLHHQHQTQGALGGSRALSNSVSNMGLSESSSLGSAKHQQQSPVSQSMQTLSDSLSGSSLYSTSANLPVMGHEKFPSDLDLDMFNGSLECDMESIIRSELMDADGLDFNFDSLISTQNVVGLNVGNFTGAKQASSQSWVPG

Approximately 95-150 kDa due to the glycosylation.

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4 or 25 mM Tris-HCL, 100 mM Glycine, pH 7.3, 10% Glycerol.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.