Frataxin/FXN 蛋白, Cynomolgus (His)

Frataxin (FXN) protein activates persulfide transfer, which is critical for [2Fe-2S] cluster assembly. It accelerates sulfur transfer from NFS1 persulfide to ISCU and small thiols, resulting in oversulfide and sulfide release. Frataxin/FXN Protein, Cynomolgus (His) is the recombinant cynomolgus-derived Frataxin/FXN protein, expressed by E. coli , with N-6*His labeled tag. The total length of Frataxin/FXN Protein, Cynomolgus (His) is 130 a.a., with molecular weight (affected by relative charge) of ~22 KDa.

Frataxin (FXN) 蛋白在核心铁硫簇 (ISC) 组装复合物内的过硫化物转移过程中充当激活剂，这对于 [2Fe-2S] 簇组装至关重要。它促进硫从 NFS1 过硫化物中间体转移到 ISCU 以及 L-半胱氨酸和谷胱甘肽等小硫醇，从而导致过硫化和硫化物释放。在 [2Fe-2S] 簇组装过程中，FXN 结合亚铁离子，并在添加 L-半胱氨酸和还原的 FDX2 后释放。ISC 组装复合物由 FXN、NFS1、LYRM4、NDUFAB1 和 FDX2 组成，启动 [2Fe-2S] 簇的从头合成，将它们转移到伴侣蛋白，如 HSCB、HSPA9 和 GLRX5。FXN 可能在防止铁催化的氧化应激中发挥作用，以其寡聚形式表现出亚铁氧化酶活性。它可能通过低聚以水铁矿矿物的形式储存大量的铁，尽管其生理相关性仍不确定。FXN 可能充当铁伴侣蛋白，保护乌头酸酶 [4Fe-4S]2+ 簇免于分解并促进酶重新激活。此外，它可能作为 FECH 的高亲和力铁结合伴侣，有助于线粒体血红素生物合成的最终步骤。FXN 调节 ACO1 的 RNA 结合活性，可能参与细胞质铁硫蛋白的生物发生，并可能有助于氧化应激抵抗和整体细胞存活。

Cynomolgus

E. coli

N-6*His

Q8HXX9 (S81-A210)

102120656  [NCBI]

SGTLGHPGSLDDTTYERLAEETLDSLAEFFEDLADKPYTFEDYDVSFGSGVLTVKLGGDLGTYVINKQTPNKQIWLSSPSSGPKRYDRTGKNWVYSHDGVSLHELLGAELTKALKTKLDLSSLAYSGKDA

Approximately 22 kDa.The reducing (R) protein migrat es as 22 kDa in SDS-PAGE may be due to relative charge.

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.