FUT8 蛋白, Human (sf9, His)

The FUT8 protein acts as a catalyst during glycosylation, specifically adding fucose in an α1-6 linkage to the first GlcNAc residue located near the N-glycan peptide chain. This enzymatic activity plays a crucial role in glycoprotein modification, contributing to the structural diversity and functional properties of N-glycans. FUT8 Protein, Human (sf9, His) is the recombinant human-derived FUT8 protein, expressed by Sf9 insect cells , with C-His labeled tag.

FUT8（或 α-1,6-岩藻糖基转移酶 8）是一种在糖基化过程中发挥关键作用的酶，通过催化将 α1-6 连接中的岩藻糖添加到 N-聚糖中肽链附近的初始 GlcNAc 残基上。这种酶活性对于糖蛋白的修饰至关重要，影响其结构和功能。通过在该 α 1-6 连接中专门添加岩藻糖，FUT8 有助于形成聚糖结构的多样性，从而影响细胞识别、信号传导和其他重要的生物过程。它必须突出 FUT8 在聚糖修饰方面的特异性，阐明其在塑造糖蛋白组和参与受糖基化影响的各种细胞功能中的作用。

Measured by its ability to hydrolyze the donor substrate GDP fucose and the specific activity is >0.75 pmoles/min/μg.

Human

Sf9 insect cells

C-His

Q9BYC5-1 (R68-K575)

2530  [NCBI]

Approximately 55 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 8.0, 10% gly.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.