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  3. Galectin-8/LGALS8 蛋白, Human (GST)

Galectin-8/LGALS8 蛋白, Human (GST)

目录号: HY-P700382
产品使用指南

Galectin-8/LGALS8蛋白是糖基化过程中的重要酶,作为β-1,3-N-乙酰氨基葡萄糖转移酶合成聚-N-乙酰乳糖胺。Galectin-8/LGALS8 对于修饰糖蛋白和糖脂至关重要,可催化 N-乙酰氨基葡萄糖转移至受体分子,并对 2 型寡糖表现出特定活性。Galectin-8/LGALS8 蛋白, Human (GST) 是重组的 Galectin-8/LGALS8 蛋白,由 E. coli 表达,带有 N-GST 标签。

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描述

Galectin-8/LGALS8 protein is an important enzyme in the glycosylation process, acting as β-1,3-N-acetylglucosaminyltransferase to synthesize poly-N-acetyllactosamine. Galectin-8/LGALS8 is essential for modifying glycoproteins and glycolipids, catalyzes the transfer of N-acetylglucosamine to acceptor molecules, and exhibits specific activity on type 2 oligosaccharides. Galectin-8/LGALS8 Protein, Human (GST) is the recombinant human-derived Galectin-8/LGALS8 protein, expressed by E. coli , with N-GST labeled tag.

研究背景

B3GNT4 是糖基化过程中的关键酶,充当 β-1,3-N-乙酰氨基葡萄糖转移酶,负责合成聚-N-乙酰乳糖胺。该酶通过催化 N-乙酰氨基葡萄糖残基转移到受体分子上,在糖蛋白和糖脂的修饰中发挥着至关重要的作用。值得注意的是,B3GNT4 对 2 型寡糖表现出特异性活性,有助于聚糖结构的多样化和复杂性。B3GNT4 合成聚-N-乙酰基乳糖胺强调了其在调节细胞相互作用中的重要性,因为聚糖结构的改变可以影响各种生物过程,包括细胞粘附、信号传导和识别事件。

种属

Human

表达系统

E. coli

标签

N-GST

蛋白编号

O00214-1 (M1-W317)

基因 ID
蛋白结构
N-term
GST
LGALS8 (M1-W317)
Accession # O00214-1
C-term
同用名
LGALS8; lectin, galactoside-binding, soluble, 8; galectin-8; galectin 8; PCTA 1; galectin-8g; Po66 carbohydrate binding protein; po66 carbohydrate-binding protein; prostate carcinoma tumor antigen 1; Gal-8; PCTA1; PCTA-1; Po66-CBP;
氨基酸序列

MMLSLNNLQNIIYNPVIPFVGTIPDQLDPGTLIVIRGHVPSDADRFQVDLQNGSSMKPRADVAFHFNPRFKRAGCIVCNTLINEKWGREEITYDTPFKREKSFEIVIMVLKDKFQVAVNGKHTLLYGHRIGPEKIDTLGIYGKVNIHSIGFSFSSDLQSTQASSLELTEISRENVPKSGTPQLRLPFAARLNTPMGPGRTVVVKGEVNANAKSFNVDLLAGKSKDIALHLNPRLNIKAFVRNSFLQESWGEEERNITSFPFSPGMYFEMIIYCDVREFKVAVNGVHSLEYKHRFKELSSIDTLEINGDIHLLEVRSW

分子量

62.8 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

Galectin-8/LGALS8 Protein, Human (GST) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Galectin-8/LGALS8 Protein, Human (GST)
目录号:
HY-P700382
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