1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. GALNT7 蛋白, Human (HEK293, His)

GALNT7 蛋白, Human (HEK293, His)

目录号: HY-P70867
COA 产品使用指南

GALNT7 蛋白是 O-连接寡糖生物合成中不可或缺的糖肽基转移酶。其酶活性涉及催化 N-乙酰基-D-半乳糖胺残基转移至预先存在的糖基化肽。GALNT7 蛋白, Human (HEK293, His) 是重组的 GALNT7 蛋白,由 HEK293 表达,带有 C-6*His 标签。GALNT7 蛋白, Human (HEK293, His) 全长 628 个氨基酸,分子量为 65-80 kDa。

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规格 价格 是否有货 数量
10 μg ¥1550 In-stock
50 μg ¥4300 In-stock
100 μg   询价  

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

The GALNT7 protein serves as an indispensable glycopeptidyl transferase for O-linked oligosaccharide biosynthesis. Its enzymatic activity involves catalyzing the transfer of N-acetyl-D-galactosamine residues to pre-existing glycosylated peptides. GALNT7 Protein, Human (HEK293, His) is the recombinant human-derived GALNT7 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of GALNT7 Protein, Human (HEK293, His) is 628 a.a., with molecular weight of 65-80 kDa.

研究背景

GALNT7 是一种糖肽转移酶,在 O-连接寡糖生物合成中发挥着至关重要的作用。与其蛋白质家族的其他成员不同,GALNT7 不充当直接将 N-乙酰基-D-半乳糖胺 (GalNAc) 转移到蛋白质受体上的丝氨酸或苏氨酸残基上的肽转移酶。相反,它催化 GalNAc 转移到已经糖基化的肽上,需要在添加额外的 GalNAc 部分之前预先在肽上添加 GalNAc。虽然 GALNT7 主要在 O-连接糖基化中发挥作用,但不能完全排除某些肽转移酶活性,并且其适当肽底物的鉴定仍然是正在进行的研究的主题。

生物活性

Measured by its ability to transfer galactose from UDP-GalNAc (HY-114365) to peptide MUC5AC-3/13 that incubate at 37℃ for 20 min. The specific activity is 1288.83 pmol/min/μg.

种属

Human

表达系统

HEK293

标签

C-6*His

蛋白编号

Q86SF2 (P30-V657)

基因 ID
蛋白结构
N-term
GALNT7 (P30-V657)
Accession # Q86SF2
6*His
C-term
同用名
PolypeptideGalNAc-transferase7; Protein-UDP acetylgalactosaminyltransferase7; UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 7; GALNT7.
氨基酸序列

PRPDDPSPLSRMREDRDVNDPMPNRGGNGLAPGEDRFKPVVPWPHVEGVEVDLESIRRINKAKNEQEHHAGGDSQKDIMQRQYLTFKPQTFTYHDPVLRPGILGNFEPKEPEPPGVVGGPGEKAKPLVLGPEFKQAIQASIKEFGFNMVASDMISLDRSVNDLRQEECKYWHYDENLLTSSVVIVFHNEGWSTLMRTVHSVIKRTPRKYLAEIVLIDDFSNKEHLKEKLDEYIKLWNGLVKVFRNERREGLIQARSIGAQKAKLGQVLIYLDAHCEVAVNWYAPLVAPISKDRTICTVPLIDVINGNTYEIIPQGGGDEDGYARGAWDWSMLWKRVPLTPQEKRLRKTKTEPYRSPAMAGGLFAIEREFFFELGLYDPGLQIWGGENFEISYKIWQCGGKLLFVPCSRVGHIYRLEGWQGNPPPIYVGSSPTLKNYVRVVEVWWDEYKDYFYASRPESQALPYGDISELKKFREDHNCKSFKWFMEEIAYDITSHYPLPPKNVDWGEIRGFETAYCIDSMGKTNGGFVELGPCHRMGGNQLFRINEANQLMQYDQCLTKGADGSKVMITHCNLNEFKEWQYFKNLHRFTHIPSGKCLDRSEVLHQVFISNCDSSKTTQKWEMNNIHSV

分子量

65-80 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

溶液.

组分

Supplied as a 0.2 μm filtered solution of 50 mM Tris-HCl, 10 mM reduced Glutathione, pH 8.0.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

N/A.

保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料

GALNT7 Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
GALNT7 Protein, Human (HEK293, His)
目录号:
HY-P70867
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