GITR Protein, Mouse (HEK293, His)

GITR (TNFRSF18) is a member of the TNFR superfamily. GITR promotes T cell activation and proliferation and increases resistance to tumors and viral infections, and exacerbates autoimmune diseases and inflammation processes^[1]. GITR Protein, Mouse (HEK293, His) is a recombinant protein with a C-terminal His label and is produced in HEK293 cells.

GITR 在调节性 T 细胞 (Treg) 和一些激活的免疫细胞上表达，包括效应 T 淋巴细胞、自然杀伤 (NK) 细胞和中性粒细胞^[1]。
GITR 的氨基酸序列人 GITR 蛋白与小鼠 GITR 蛋白同源性较低。
GITR 不具有任何酶活性，信号传导是通过招募 TRAF 家族成员（特别是 TRAF1、TRAF2 和 TRAF5）至 GITR 信号复合物来传播的。然后信号通过 NF-kB 和 MAPK 途径介导。GITR 不具有任何酶活性，信号传导是通过将 TRAF 家族成员（特别是 TRAF1、TRAF2 和 TRAF5）招募到 GITR 信号传导复合物来传播的。然后，信号通过 NF-kB 和 MAPK 通路介导，保护 T 细胞免受 TCR 激活诱导的细胞死亡^[2]。
GITR（糖皮质激素诱导的 TNFR 相关蛋白，也称为TNFRSF18）是一种 I 型跨膜蛋白。GITR 刺激效应 T 淋巴细胞增殖并部分逆转 CD4+CD25+ Tregs^[1] 的免疫抑制功能。GITR 被其配体 GITRL (TNFSF18) 激活。GITR 以时间和剂量依赖性方式诱导小鼠巨噬细胞中的 NOS^[3]。GITR 可在体外和体内抑制多发性骨髓瘤 (MM) 细胞增殖并诱导细胞凋亡^[4]。

GITR (mouse; .1, .1, .2, .5 µg/mL; -46 h) increases the generation of nitric oxide in a dose and time-dependent manner in mouse macrophage RAW 264.7 cells^[3].

GITR overexpressing cells are injected into SCID-bg mice via tail, showing a significant reduction of growth of MM cells in mice after 4 weeks in bone marrow tissues^[4].

Measured by its binding ability in a functional ELISA. Immobilized Mouse GITR at 2 μg/mL (100 μL/well) can bind Biotinylated Human GITR Ligand. The ED₅₀ for this effect is 16.44 ng/mL.

• 
  Measured by its binding ability in a functional ELISA. Immobilized Mouse GITR at 2 μg/mL (100 μL/well) can bind Biotinylated Human GITR Ligand. The ED₅₀ for this effect is 16.44 ng/mL.

Mouse

HEK293

C-6*His

O35714-1/NP_033426.1 (S22-Q150)

21936  [NCBI]

SVVEEPGCGPGKVQNGSGNNTRCCSLYAPGKEDCPKERCICVTPEYHCGDPQCKICKHYPCQPGQRVESQGDIVFGFRCVACAMGTFSAGRDGHCRLWTNCSQFGFLTMFPGNKTHNAVCIPEPLPTEQ

Approximately 28-33 kDa due to the glycosylation

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Tian J, et al. The Role of GITR/GITRL Interaction in Autoimmune Diseases. Front Immunol. 2020 Oct 9;11:588682.  [Content Brief]

  [2]. Krausz LT, et al. GITR-GITRL system, a novel player in shock and inflammation. ScientificWorldJournal. 2007 May 1;7:533-66.  [Content Brief]

  [3]. Shin HH, et al. Recombinant glucocorticoid induced tumor necrosis factor receptor (rGITR) induces NOS in murine macrophage. FEBS Lett. 2002 Mar 13;514(2-3):275-80.  [Content Brief]

  [4]. Liu Y, et al. Novel tumor suppressor function of glucocorticoid-induced TNF receptor GITR in multiple myeloma. PLoS One. 2013 Jun 13;8(6):e66982.  [Content Brief]