GPR114 蛋白, Human (HEK293, Fc)

GPR114, an adhesion G protein-coupled receptor (GPCR), crucially activates the adenylate cyclase pathway by coupling with G(s) subunit alpha. Isoform 1, displaying constitutive activity, elevates basal cyclic AMP (cAMP) levels and responds to mechanical stimulation, emphasizing GPR114's functional significance in intracellular signaling cascades and its role in cellular responses to mechanical cues. GPR114 Protein, Human (HEK293, Fc) is the recombinant human-derived GPR114 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of GPR114 Protein, Human (HEK293, Fc) is 163 a.a., with molecular weight of 57-75 kDa.

GPR114 是一种粘附 G 蛋白偶联受体 (GPCR)，通过与鸟嘌呤核苷酸结合蛋白的 G(s) 亚基 α 偶联并激活腺苷酸环化酶途径，在信号转导中发挥关键作用。值得注意的是，异构体 1 表现出组成型活性，导致环 AMP (cAMP) 基础水平升高，并表现出对机械刺激（例如摇动）的反应性。这凸显了 GPR114 在介导细胞内信号级联中的功能意义，并表明它参与了细胞对机械信号的反应。

Measured by the ability of the immobilized protein to enhance the adhesion of U251 human glioblastoma cells to human Fibronectin. When 4 x 10⁴ cells/well are added to plates coated with human Fibronectin (0.1 µg/mL) and rhGPR114 (10µg/well, 100µL/well) for 45 minutes at 37 °C, there is a 2.1 fold increase in adhesion to human fibronectin induced by the presence of rhGPR114.

Human

HEK293

C-hFc

Q8IZF4 (E22-G184)

221188  [NCBI]

ETWEELLSYMENMQVSRGRSSVFSSRQLHQLEQMLLNTSFPGYNLTLQTPTIQSLAFKLSCDFSGLSLTSATLKRVPQAGGQHARGQHAMQFPAELTRDACKTRPRELRLICIYFSNTHFFKDENNSSLLNNYVLGAQLSHGHVNNLRDPVNISFWHNQSLEG

Approximately 57-75 kDa due to the glycosylation.

Greater than 90% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4, 10% trehalose, 0.02% Tween80.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.