MIP-2/CXCL2 蛋白, Mouse (His-SUMO)

CXCL2, also called Gro-beta or MIP-2, is a pro-inflammatory cytokine with chemotactic activities on neutrophils. CXCL2 is produced by activated monocytes and neutrophils and expressed at sites of inflammation. CXCL2 is involved in many immune responses including wound healing, cancer metastasis, and angiogenesis^[1][2]. MIP-2/CXCL2 Protein, Mouse (His-SUMO) is produced in E. coli with a N-Terminal His-tag and a N-Terminal SUMO-tag. It consists of 73 amino acids (A28-N100).

CXCL2 是一种由内毒素诱导的趋化因子，可作为中性粒细胞的极其有效的化学引诱剂，充当重要的炎症介质。CXCL2 可以由多种不同的细胞类型产生，包括巨噬细胞和癌细胞。CXCL2 参与癌症转移、血管生成和伤口愈合^[1][4][5]。
人CXCL2蛋白序列与小鼠和大鼠CXCL2蛋白的同源性较低。
CXCL2与相关趋化因子CXCL1的氨基酸序列有90%相同。CXCL2 基因位于人类 4 号染色体上的其他 CXC 趋化因子簇中。CXCL2 与巨噬细胞、中性粒细胞和上皮细胞上表达的 G 蛋白偶联受体 CXCR2 (IL-8RB) 结合，其经典功能是充当趋化因子，将中性粒细胞吸引至损伤部位^{[2] [3]}。
在血小板激活因子诱导的休克和肠损伤模型中，LPS 会诱导 CXCL2 表达并促进中性粒细胞迁移。在急性肺损伤中，CXCR2 配体（包括 CXCL1/2/3）对多形核白细胞具有趋化作用^[4]。CXCL2 可以在体外引起结直肠肿瘤细胞迁移的剂量依赖性增加。此外，根据 Bachmeier 等人的研究，CXCL-1 和 -2 沉默可以下调多个促转移基因并抑制乳腺癌细胞的转移潜力^[5]。

Determined by its ability to chemoattract BaF3 mouse pro-B cells transfected with Human CXCR2 .The ED₅₀ for this effect is 4.143 ng/mL, corresponding to a specific activity is 2.41×10⁵ U/mg.

Mouse

E. coli

N-His;N-SUMO

P10889 (A28-N100)

20310  [NCBI]

Approximately 30 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 0.1% TFA, pH 2.9. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Laila A Al-Alwan, et al. Differential roles of CXCL2 and CXCL3 and their receptors in regulating normal and asthmatic airway smooth muscle cell migration. J Immunol. 2013 Sep 1;191(5):2731-41.  [Content Brief]

  [2]. Louis M Pelus, et al. Peripheral blood stem cell mobilization: the CXCR2 ligand GRObeta rapidly mobilizes hematopoietic stem cells with enhanced engraftment properties. Exp Hematol. 2006 Aug;34(8):1010-20.  [Content Brief]

  [3]. Aimalie L Hardaway, et al. Marrow adipocyte-derived CXCL1 and CXCL2 contribute to osteolysis in metastatic prostate cancer. Clin Exp Metastasis. 2015 Apr;32(4):353-68.  [Content Brief]

  [4]. Jeongim Ha, et al. CXCL2 mediates lipopolysaccharide-induced osteoclastogenesis in RANKL-primed precursors. Cytokine. 2011 Jul;55(1):48-55.  [Content Brief]

  [5]. Yu Lu, et al. Type conversion of secretomes in a 3D TAM2 and HCC cell co-culture system and functional importance of CXCL2 in HCC. Sci Rep. 2016 Apr 27;6:24558.  [Content Brief]

  [6]. Sagar Paudel, et al. CXCL1 regulates neutrophil homeostasis in pneumonia-derived sepsis caused by Streptococcus pneumoniae serotype 3. Blood. 2019 Mar 21;133(12):1335-1345.  [Content Brief]