1. 重组蛋白
  2. Enzymes & Regulators
  3. Oxidoreductases (EC 1)
  4. HAAO 蛋白, Human (sf9, His-GST)

HAAO 蛋白, Human (sf9, His-GST)

目录号: HY-P76962
产品使用指南

HAAO 蛋白在细胞代谢中发挥关键作用,催化 3-羟基邻氨基苯甲酸的氧化开环。这导致形成 2-氨基-3-羧基粘康酸半醛,其自发环化为喹啉酸。HAAO 蛋白, Human (sf9, His-GST) 是重组的 HAAO 蛋白,由 Sf9 insect cells 表达,带有 C-His, N-GST 标签。HAAO 蛋白, Human (sf9, His-GST) 全长 286 个氨基酸,分子量约为 ~60.4 KDa。

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描述

HAAO proteins play a key role in cellular metabolism, catalyzing the oxidative ring-opening of 3-hydroxyanthranilic acid. This leads to the formation of 2-amino-3-carboxymuconic acid semialdehyde, which spontaneously cyclizes to quinolinic acid. HAAO Protein, Human (sf9, His-GST) is the recombinant human-derived HAAO protein, expressed by Sf9 insect cells , with C-His, N-GST labeled tag. The total length of HAAO Protein, Human (sf9, His-GST) is 286 a.a., with molecular weight of ~60.4 KDa.

研究背景

HAAO 蛋白通过催化 3-羟基邻氨基苯甲酸的氧化开环,导致形成 2-氨基-3-羧基粘康酸半醛,随后进行自发环化生成喹啉酸,从而在细胞代谢中发挥关键作用。这种酶促过程是色氨酸分解代谢不可或缺的一部分,并有助于产生喹啉,喹啉是一种参与多种生物途径的化合物。HAAO 蛋白在介导这些生化反应中的功能凸显了其在调节 3-羟基邻氨基苯甲酸代谢命运中的重要性,并强调了其在细胞稳态中更广泛的意义。

种属

Human

表达系统

Sf9 insect cells

标签

C-His;N-GST

蛋白编号

P46952 (M1-G286)

基因 ID
蛋白结构
N-term
His-GST
HAAO (M1-G286)
Accession # P46952
C-term
同用名
3-hydroxyanthranilate 3,4-dioxygenase; 3-HAO; HAD
分子量

Approximately 60.4 kDa.

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

HAAO Protein, Human (sf9, His-GST) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
HAAO Protein, Human (sf9, His-GST)
目录号:
HY-P76962
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