HCoV-OC43 Spike/S2 蛋白 (AVR40344, sf9, His)

Coronaviruses, enveloped positive-strand RNA viruses, generally cause mild to moderate upper-respiratory tract illness. OC43, like SARS-CoV-2, is in the genus Betacoronavirus but grouped in another subgenus called Embecovirus. The NS2 protein of HCoV-OC43 has cyclic phosphodiesterase activity, which may modulate cAMP-mediated signaling and important physiological processes such as lipid metabolism and apoptosis. HCoV-OC43 replicate principally in the upper respiratory tract epithelial cells, where they produce virus and cause local respiratory symptoms^[1][2]. HCoV-OC43 Spike/S2 Protein (AVR40344, sf9, His) is the recombinant Virus-derived HCoV-OC43 Spike/S2 protein, expressed by Sf9 insect cells , with C-His labeled tag.

迄今为止已鉴定出七种人类冠状病毒 (HCoV)，即HCoV-229E、HCoV-OC43、HCoV-NL63、HCoV-HKU1、严重急性呼吸综合征冠状病毒 (SARS-CoV)、中东呼吸综合征冠状病毒 (MERS-CoV) 和新型冠状病毒 (2019-nCoV，又名 SARS-CoV-2)。M 蛋白 (25-30 kDa) 是最丰富的结构蛋白，具有三个跨膜结构域。HCoV-OC43 和一些动物冠状病毒 (包括小鼠肝炎病毒 (MHV) 和牛冠状病毒 (BCoV)) 中 M 蛋白的短 N 端胞外域经过 O 连接糖基化修饰。HCoV-OC43 ns12.9 蛋白是最近发现的一种病毒孔蛋白，可促进病毒颗粒的形态发生和发病机制。HCoV-OC43 感染会激活 IRE1 并诱导 X-box 蛋白 1 (XBP1) mRNA 剪接，从而上调下游 UPR 效应基因。在 HCoV-OC43 的 S 蛋白中引入两个点突变 (H183R 和 Y241H) 会诱导更高程度的 XBP1 mRNA 剪接，并导致更明显的细胞凋亡。HCoV-OC43 结构蛋白或辅助蛋白的过度表达还会导致 30 多个与先天免疫反应相关的基因下调，包括编码 MAP 激酶、Toll 样受体、干扰素、白细胞介素和信号转导蛋白的基因。与 SARS-CoV 和 MERS-CoV 的 PLPro 类似，HCoV-NL63 的 PLP2 具有去泛素化 (DUB) 活性^[1]。

Virus

Sf9 insect cells

C-His

AVR40344 (A766-P1304)

/

Approximately 60.7 kDa.

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.