1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. HDHD2 蛋白, Human (His)

HDHD2 蛋白, Human (His)

目录号: HY-P70912
产品使用指南

卤代酸脱卤酶样水解酶域蛋白 2 (HDHD2) 具有水解酶和磷酸酶活性。HDHD2 在小脑中有表达,其蛋白修饰改变可能与抑郁症的发生有关。HDHD2 与核糖体蛋白复合物密切相关,可调节核糖体蛋白的合成,可能通过激活核糖体活性发挥神经保护作用。HDHD2 蛋白, Human (His) 是重组的 HDHD2 蛋白,由 E. coli 表达,带有 N-6*His 标签。

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描述

Haloacid dehalogenase-like hydrolase domain-containing protein 2 (HDHD2) has hydrolase and phosphatase activities. HDHD2 is expressed in cerebellum and shift of HDHD2 modifications might be relative with depression.HDHD2 is well associated with ribosomal protein complex and regulates the protein synthesis and might has a neuroprotective function via activating the ribosomal activities. HDHD2 Protein, Human (His) is the recombinant human-derived HDHD2 protein, expressed by E. coli , with N-6*His labeled tag.

研究背景

卤代酸脱卤酶样水解酶域蛋白 2 (HDHD2) 是卤代酸脱氢酶 (HAD) 样超家族的成员之一,具有水解酶和磷酸酶活性。HDHD2 在小脑中有表达,其蛋白修饰改变可能与抑郁症的发生有关。HDHD2 与核糖体蛋白复合物密切相关,可调节核糖体蛋白的合成,可能通过激活核糖体活性发挥神经保护作用[1][2]

种属

Human

表达系统

E. coli

标签

N-6*His

蛋白编号

Q9H0R4-1 (M1-L259)

基因 ID
蛋白结构
N-term
6*His
HDHD2 (M1-L259)
Accession # Q9H0R4-1
C-term
同用名
Haloacid Dehalogenase-Like Hydrolase Domain-Containing Protein 2; HDHD2
氨基酸序列

MAACRALKAVLVDLSGTLHIEDAAVPGAQEALKRLRGASVIIRFVTNTTKESKQDLLERLRKLEFDISEDEIFTSLTAARSLLERKQVRPMLLVDDRALPDFKGIQTSDPNAVVMGLAPEHFHYQILNQAFRLLLDGAPLIAIHKARYYKRKDGLALGPGPFVTALEYATDTKATVVGKPEKTFFLEALRGTGCEPEEAVMIGDDCRDDVGGAQDVGMLGILVKTGKYRASDEEKINPPPYLTCESFPHAVDHILQHLL

分子量

Approximately 30.0 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

HDHD2 Protein, Human (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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HDHD2 Protein, Human (His)
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HY-P70912
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