IFN-gamma 蛋白, Sheep (His)

IFN-gamma (interferon-gamma) is a type II interferon produced by T cells and NK cells, which can significantly activate antibacterial, antiviral and antitumor responses. It acts through the JAK-STAT pathway and its receptor IFNGR1 to affect gene regulation. IFN-gamma Protein, Sheep (His) is the recombinant sheep-derived IFN-gamma protein, expressed by E. coli , with N-6*His labeled tag. The total length of IFN-gamma Protein, Sheep (His) is 143 a.a., with molecular weight of ~21 kDa.

IFN-gamma（干扰素-gamma）是一种由 T 细胞和 NK 细胞等免疫细胞产生的 II 型干扰素，通过激活效应免疫细胞和增强抗原呈递，在抗菌、抗病毒和抗肿瘤反应中发挥关键作用。其主要信号传导通过 JAK-STAT 途径与其受体 IFNGR1 相互作用而发生，从而影响基因调控。IFN-γ 结合后，IFNGR1 胞内结构域展开，促进下游信号成分（包括 JAK2、JAK1 和 STAT1）的关联。该序列导致 STAT1 激活、核易位和 IFN-γ 调节基因的转录，其中许多基因编码 IRF1 等转录因子，能够进一步驱动后续转录事件的调节。IFN-gamma 还通过诱导免疫蛋白酶体亚基替换催化蛋白酶体亚基，从而促进 I 类抗原呈递途径，从而增加 I 类 MHC 负载的肽的数量、质量和库。此外，它还通过诱导激活剂 PA28 的表达来提高肽生成的效率，PA28 与蛋白酶体结合并改变其蛋白水解切割偏好。此外，IFN-γ 通过促进组织蛋白酶 B/CTSB、H/CTSH 和 L/CTSL 等关键分子的表达，上调细胞表面的 MHC II 复合物。除了其免疫功能外，IFN-γ 还参与造血干细胞在发育过程中和稳态条件下的调节，影响其发育、静止和分化。IFN-γ 作为同二聚体存在，通过其胞外结构域与 IFNGR1 相互作用，这是一种促进 IFNGR1 二聚化以协调其在免疫反应和造血中多样化且关键功能的关键相互作用。

Sheep

E. coli

N-6*His

P17773 (Q24-M166)

443396  [NCBI]

QGPFFKEIENLKEYFNASNPDVAKGGPLFSEILKNWKEESDKKIIQSQIVSFYFKLFENLKDNQVIQRSMDIIKQDMFQKFLNGSSEKLEDFKRLIQIPVDDLQIQRKAINELIKVMNDLSPKSNLRKRKRSQNLFRGRRASM

Approximately 21 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm solution of 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.