IL-33 蛋白, Canine

IL-33 Protein activates NF-kappa-B and MAPK signaling, inducing Th2 cell maturation and cytokine secretion. It activates mast cells, basophils, eosinophils, and natural killer cells, acting as a chemoattractant for Th2 cells. IL-33 preserves Krebs cycle integrity, reducing reactive oxygen species production. In quiescent endothelial cells, IL-33 acts as a transcriptional repressor, but is lost upon angiogenic or pro-inflammatory activation. IL-33 Protein, Canine is the recombinant canine-derived IL-33 protein, expressed by E. coli , with N-Met labeled tag. The total length of IL-33 Protein, Canine is 154 a.a., with molecular weight of ~19 kDa.

IL-33 蛋白是一种细胞因子，可结合并激活 IL1RL1/ST2 受体，从而激活靶细胞中的 NF-kappa-B 和 MAPK 信号通路。它在 Th2 细胞的成熟中发挥作用，并诱导 T 辅助细胞 2 型相关细胞因子的分泌。IL-33 还参与肥大细胞、嗜碱性粒细胞、嗜酸性粒细胞和自然杀伤细胞的激活。它充当 Th2 细胞的化学引诱剂，并可能充当“警报器”，在组织损伤期间放大免疫反应。此外，IL-33 通过 UCP2 诱导线粒体快速重新布线，减少活性氧的产生并保持克雷布斯循环的完整性，这对于衣康酸的产生以及随后解决炎症的替代激活巨噬细胞的分化至关重要。在静止的内皮细胞中，IL-33以其未切割的形式组成型和丰富地表达，作为具有转录抑制特性的染色质相关核因子并隔离核NF-kappaB/RELA，导致其靶基因的表达较低。然而，这种形式在血管生成或促炎激活后迅速消失。

Measured by its binding ability in a functional ELISA. Immobilized IL-33 Protein, Canine at 10 μg/mL (100 μL/well) can bind human IL1R4-Fc and the EC₅₀ is 0.25-0.66 μg/mL.

Canine

E. coli

Tag Free

O97863 (S110-S263)

403810  [NCBI]

SIQEYSASLSTYNDQSITFVFEDGSYEIYVEDLRKGQEKDKVLFRYYDSQSPSHETGDDVDGQTLLVNLSPTKDKDFLLHANNEEHSVELQKCENQLPDQAFFLLHRKSSECVSFECKNNPGVFIGVKDNHLALIKVGDQTKDSYIEKTIFKLS

Approximately 19 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.