1. 重组蛋白
  2. Cytokines and Growth Factors
  3. Interleukin & Receptors
  4. IL-33
  5. IL-33 蛋白, Cynomolgus

IL-33 蛋白, Cynomolgus

目录号: HY-P73883
COA 产品使用指南

IL-33 蛋白是属于 IL-1 超家族的细胞因子。IL-33 诱导辅助 T 细胞、肥大细胞、嗜酸性粒细胞和嗜碱性粒细胞产生 2 型细胞因子。IL-33 在细胞内作为核因子发挥作用,在细胞外作为细胞因子发挥作用。IL-33 通过 IL-1 受体 ST 2 介导其生物效应,激活 NF-kappaB 和 MAP 激酶,并驱动体外极化 T(H)2 细胞产生 T(H)2 相关细胞因子。IL-33 蛋白, Cynomolgus 是重组的 IL-33 蛋白,由 E. coli 表达,不带标签。

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10 μg ¥950 In-stock
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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

IL-33 Protein is a cytokine belonging to the IL-1 superfamily. IL-33 induces helper T cells, mast cells, eosinophils and basophils to produce type 2 cytokines. IL-33 acts intracellularly as a nuclear factor and extracellularly as a cytokine. IL-33 mediates its biological effects via IL-1 receptor ST 2, activates NF-kappaB and MAP kinases, and drives production of T(H)2-associated cytokines from in vitro polarized T(H)2 cells. IL-33 Protein, Cynomolgus is the recombinant cynomolgus-derived IL-33 protein, expressed by E. coli , with tag free.

研究背景

白细胞介素 33 (IL-33) 是属于 IL-1 超家族的细胞因子,主要来源包括人血管树内皮细胞和小鼠外膜基质细胞、屏障组织中的上皮细胞、淋巴器官中的成纤维细胞网状细胞 (FRC)、以及神经系统中的神经胶质细胞、神经元和星形胶质细胞。IL-33 诱导辅助 T 细胞、肥大细胞、嗜酸性粒细胞和嗜碱性粒细胞产生 2 型细胞因子。IL-33 在细胞内作为核因子发挥作用,在细胞外作为细胞因子发挥作用。作为一种细胞因子,IL-33 通过 IL-1 受体 ST 2 介导其生物效应,激活 NF-kappaB 和 MAP 激酶,并驱动体外极化 T(H)2 细胞产生 T(H)2 相关细胞因子。在体内,IL-33 诱导 IL-4、IL-5 和 IL-13 的表达,导致粘膜器官发生严重的病理变化。细胞应激或暴露于炎症可以增强或诱导 IL-33 的细胞表达。IL-33 表现出多效性功能,支持 IFN-γ、IL-9 和 IL-17 主导的免疫反应,作为宿主对病原体或免疫介导的炎症性疾病反应的一部分[1][2]

生物活性

Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus IL-33 Protein is immobilized at 5 µg/mL (100 µL/well) can bind Recombinant Human IL1RL1 Protein. The ED50 for this effect is 55.8 ng/mL.

  • Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus IL-33 Protein is immobilized at 5 µg/mL (100 µL/well) can bind Recombinant Human IL1RL1 Protein. The ED50 for this effect is 55.8 ng/mL.
种属

Cynomolgus

表达系统

E. coli

标签

Tag Free

蛋白编号

A0A2K5W3I1/XP_005581824 (S112-I270)

基因 ID
蛋白结构
N-term
IL-33 (S112-I270)
Accession # A0A2K5W3I1/XP_005581824
C-term
同用名
Interleukin-33; IL-33; IL-1F11; NF-HEV; DVS 27
氨基酸序列

SITGISPITESLASLSTYNDQSITFALEDESYEIYVEDLKKDKKKDKVLLSYYESQHPSSESGDGVDGKMLMVTLSPTKDFWLQANNKEHSVELHKCEKPLPDQAFFVLHNRSFNCVSFECKTDPGVFIGVKDNHLALIKVDYSENLGSENILFKLSEI

分子量

Approximately 18.1 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

IL-33 Protein, Cynomolgus 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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