IL-4 蛋白, Rhesus macaque

IL-4 protein actively engages in B-cell activation, serving as a costimulator for DNA synthesis, inducing class II MHC molecule expression, and enhancing IgE and IgG1 secretion. It regulates CD23 expression on lymphocytes and monocytes, positively influences IL31RA expression in macrophages, and induces autophagy in dendritic cells through mTORC1 signaling interference and RUFY4 induction. IL-4 Protein, Rhesus macaque is the recombinant Rhesus Macaque-derived IL-4 protein, expressed by E. coli , with tag free.

IL-4 蛋白积极参与各种 B 细胞激活过程和其他细胞类型，充当 DNA 合成的共刺激剂。此外，它还能诱导静息 B 细胞表达 II 类 MHC 分子，并增强 IgE 和 IgG1 的分泌和细胞表面表达。IL-4 还在淋巴细胞和单核细胞上 IgE 低亲和力 Fc 受体 (CD23) 的表达中发挥调节作用。此外，它还可以通过干扰 mTORC1 信号传导和诱导 RUFY4 来正向调节巨噬细胞中 IL31RA 的表达并刺激树突状细胞中的自噬。

The ED₅₀ as determined by a cell proliferation assay using human TF-1 cells is less than 1.0 ng/mL, corresponding to a specific activity of >1.0x10⁶ IU/mg.

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  Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED₅₀ for this effect is 0.2042 ng/mL, corresponding to a specific activity is 4.897×10⁶ U/mg

Rhesus Macaque

E. coli

Tag Free

P51492 (H25-S153)

574281  [NCBI]

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Approximately 14.9 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm solution of 2 xPBS, 5 % Trehalose, pH 7.4 or PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.